4.6 Article

A mammalian messenger RNA sex determination method from humpback whale (Megaptera novaeangliae) blubber biopsies

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Article Multidisciplinary Sciences

A mammalian messenger RNA sex determination method from humpback whale (Megaptera novaeangliae) blubber biopsies

Jacob M. J. Linsky et al.

Summary: This article introduces a protocol for RNA extraction from blubber tissue and a novel mammalian RNA sex determination method. By using duplex reverse transcription-quantitative polymerase chain reaction (RT-qPCR), the individual's sex can be determined based on gene expression measurements, which is of great significance in future cetacean health studies.

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