Preparation of biopolymer pectin fascinate hydroxyapatite nanocomposite for biomedical applications

The synthesis of pectin-mediated HAP Nanoparticle (pHAP) and Hydroxyapatite/Pectin Nanocomposite (HAP/P Composite) utilizing carrot pomace pectin as a template to support greener technologies was the focus of this research. To produce a regulated crystallinity, pectin is extracted from carrot pomace. Spectral techniques such as Fourier transform infrared spectroscopy (FTIR) to find out the chemical behavior of the compound, proton-1 nuclear resonance spectroscopy (1H NMR), and carbon-13 nuclear magnetic resonance spectroscopy (13C NMR) to identify the H and C atoms that were in the extricated pectin. FTIR, SEM, and XRD were used to assess the crystallinity, shape, and purity of the HAP nanoparticles. A protein denaturation assay and a proteinase inhibition assay were used to assess the anti-inflammatory effect of pectin-mediated HAP nanoparticles and Hydroxyapatite/Pectin Nanocomposite. According to the results, the pectin-mediated HAP nanoparticles generated in the presence of an optimal pectin concentration are pure, spherical, low crystalline, and discrete particles with a reduced size. Furthermore, it has antimicrobial properties. According to this study, the HAP nanoparticles incorporated utilizing pectin as a green template can operate as a useful biomaterial for biomedical purposes. MTT Assay and ALP Activity were performed on pHAP and HAP/Pectin Nanocomposites. When compared to pectin-mediated hydroxyapatite, the experimental results demonstrated that HAP/Pectin Nanocomposites have higher cell survival and bone mineralization. As a result, the HAP/Pectin Nanocomposites could be useful in biomedical applications.

Automated summary

The synthesis of pectin-mediated HAP nanoparticles and HAP/pectin nanocomposites using carrot pomace pectin as a template was the focus of this study. The results showed that the nanoparticles produced at the optimal pectin concentration were pure, spherical, low crystalline, and discrete particles with reduced size, and had antimicrobial properties. In comparison to pectin-mediated hydroxyapatite, the HAP/pectin nanocomposites exhibited higher cell survival and bone mineralization, indicating their potential for biomedical applications.