期刊
FRONTIERS IN GENETICS
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2022.1009220
关键词
domestic cat (Felis catus); microRNA; ovary; testis; RNA-seq
资金
- Smithsonian Scholarly Studies Awards Program
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brazilian Federal Agency for the Support and Evaluation of Graduate Education)
- PDSE (Programa de Doutorado Sanduiche no Exterior-internship program PhD abroad)
This study aimed to analyze miRNAs in ovarian and testicular tissues of domestic cats, with a focus on their potential involvement in gonadal function, testis development, and cellular stress response. By sequencing miRNAs, the researchers identified miRNA precursors expressed in cat gonads and discovered novel candidate precursors. They also found associations between miRNAs and mRNAs, highlighting their potential role in testicular development and spermatogenesis.
The aim of the study was to perform the first in-depth analysis of miRNAs in ovarian and testicular tissues of the domestic cat, a critical biomedical model. Specifically, potential miRNA involvement was explored in gonadal function, testis development, and cellular stress response to preservation protocols. We performed miRNA-sequencing on 20 ovarian and 20 testicular samples from 15 cats, including different ages and tissue treatments. Using fresh tissues (n = 15), we confirmed gonadal expression of 183 miRNA precursors and discovered additional 52 novel feline candidate precursors. We integrated the mRNA data from our previous study on the same age and treatment groups to create in-silico miRNA-mRNA networks and their functional enrichment, which allows comprehensive exploration into possible miRNA functions in cat gonads. Clusters of miRNAs united by shared differentially expressed mRNA targets are potentially involved in testicular development and spermatogenesis. MicroRNAs could play a significant role in ovarian tissue response to stress from microwave-assisted dehydration, with smaller roles in cellular response to vitrification in both ovary and testis. This new list of miRNAs with potential function in cat gonads is a major step towards understanding the gonadal biology, as well as optimizing fertility preservation protocols.
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