期刊
FRONTIERS IN GENETICS
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2022.918728
关键词
miRNA; microRNA; chronic intermittent hypoxia (CIH); kidney injury; miRNA sequencing; bioinformatics analysis
资金
- Science and Technology Project of Fujian Education Department
- Natural Science Foundation of Fujian Province
- Young and Middle-Aged Backbone Talents Training Project of Fujian Provincial Health Commission
- Science and Technology Projects of Quanzhou
- Startup Fund for Scientific Research of Fujian Medical University
- Doctoral Miaopu Project of the Second Affiliated Hospital of Fujian Medical University
- [JAT200154]
- [JAT200136]
- [2022J01274]
- [2021GGA040]
- [2021C054R]
- [2021QH1118]
- [BS202115]
This study investigated the expression profiles of miRNAs in a mouse model of chronic intermittent hypoxia-induced renal injury using miRNA sequencing. A total of 18 differentially expressed miRNAs were identified. The analysis also revealed the potential involvement of the Wnt signaling pathway in the development of this pathology.
Background: miRNAs have been reported to participate in various diseases. Nevertheless, the expression patterns of miRNA in obstructive sleep apnea (OSA)-induced kidney injury remain poorly characterized. In the current study, miRNA sequencing (miRNA-seq) was conducted to investigate miRNA expression profiles in a chronic intermittent hypoxia (CIH)-induced renal injury mouse model. Methods: The mouse model of chronic intermittent hypoxia was established. Differentially expressed miRNAs (DEmiRs) were detected using miRNA-seq technology. The sequencing data were subjected to Gene Ontology (GO) functional enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses using a bioinformatics approach. RT-qPCR was further used to evaluate the sequencing results. Finally, we created a network for clarifying the relationship between the miRNAs and target genes. Results: In total, nine miRNAs were identified to be upregulated and nine to be downregulated in a mouse model of renal injury induced by chronic intermittent hypoxia. The Kyoto Encyclopedia of Genes and Genomes analyses revealed that the Wnt signaling pathway was involved in the development of chronic intermittent hypoxia-induced renal injury. Subsequently, eight DEmiRs, namely, mmu-miR-486b-3p, mmu-miR-215-5p, mmu-miR-212-3p, mmu-miR-344-3p, mmu-miR-181b-1-3p, mmu-miR-467a-3p, mmu-miR-467 d-3p, and mmu-miR-96-5p, showed a similar trend of expression when verified using RT-qPCR. Finally, five selected DEmiRs were used to construct a miRNA-mRNA network. Conclusion: In conclusion, a total of 18 DEmiRs were identified in the mouse model of chronic intermittent hypoxia-induced renal injury. These findings advance our understanding of the molecular regulatory mechanisms underlying the pathophysiology of obstructive sleep apnea-associated chronic kidney disease.
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