期刊
FRONTIERS IN NEUROLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fneur.2022.986377
关键词
peripheral nerve injury; decellularized tissue; bio-scaffolds; PC-12 cells; ECM
资金
- Zhejiang Medical Science and Technology Project
- National Key Research and Development Program of China
- National Natural Science Foundation of China
- [2022KY1101]
- [2019KY569]
- [2022KY311]
- [2018YFA0703000]
- [52075482]
This study explores the potential of decellularized kidney and decellularized peripheral nerve as replacements for nerve repair and regeneration. The results show that while the cell behaviors are similar, the arrangement of cells on the decellularized peripheral nerve scaffold is more regular. Furthermore, the expression of axon growth-associated genes and proteins is significantly upregulated in the decellularized kidney group, which also contains higher levels of growth factors. This suggests that decellularized kidney may have a potential application in promoting nerve repair and regeneration.
Peripheral nerve injuries cause an absence or destruction of nerves. Decellularized nerves, acting as a replacement for autografts, have been investigated in the promotion of nerve repair and regeneration, always being incorporated with stem cells or growth factors. However, such a strategy is limited by size availability. The potential application in heterotopic transplantation of other decellularized tissues needs to be further explored. In this study, rat decellularized kidney (dK) was selected to be compared with decellularized peripheral nerve (dN), since dK has aboundant ECM components and growth factors. The PC-12 cells were cultured on dK and dN scaffolds, as shown in the similar behaviors of cell metabolism and viability, but have a more regular arrangement on dN compared to dK, indicating that the natural structure plays an important role in guiding cell extension. However, we found significant upregulation of axon-growth-associated genes and proteins of PC-12 cells in the dK group compared to the dN group by qRT-PCR, immunofluorescence, and western blotting. Furthermore, various neurotrophic factors and growth factors of acellular kidney and nerve were evaluated by ELISA assay. The lower expression of neurotrophic factors but higher expression of growth factors such as VEGF and HGF from dK suggests that axon growth and extension for PC-12 cells may be partially mediated by VEGF and HGF expression from decellularized kidney, which further points to a potential application in nerve repair and regeneration.
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