期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.911806
关键词
HIV-1; CRF07_BC; CXCR4; CCR5; tropism
类别
资金
- Projects of International Cooperation from the Ministry of Science and Technology of China [2016YFE0107600]
- Science Priority Grant from the State Key Laboratory of Infections Disease Prevention and Control [2019SKLID602]
- National Science and Technology Major Project of China [2018ZX10721102-006]
CRF07_BC is a prevalent HIV-1 strain in China, characterized by predominantly single CCR5 tropism. This study found evidence that CRF07_BC has the ability to evolve into CXCR4 strains.
CRF07_BC is one of the most prevalent HIV-1 strains in China, which contributes over one-third of the virus transmissions in the country. In general, CRF07_BC is associated with slower disease progression, while the underlying mechanisms remain unclear. Our study focused on envelope proteins (Env) and its V3 loop which determine viral binding to co-receptors during infection of cells. We studied a large dataset of 3,937 env sequences in China and found that CRF07_BC had a unique profile of predominantly single CCR5 tropism compared with CCR5 and CXCR4 dual tropisms in other HIV-1 subtypes. The percentages of the CXCR4-tropic virus in B (3.7%) and CRF01_AE (10.4%) infection are much higher than that of CRF07_BC (0.1%), which is supported by median false-positive rates (FPRs) of 69.8%, 25.5%, and 13.4% for CRF07_BC, B, and CRF01_AE respectively, with a cutoff FPR for CXCR4-tropic at 2%. In this study, we identified the first pure CXCR4-tropic virus from one CRF07_BC-infected patient with an extremely low CD4(+)T cell count (7 cells/mm(3)). Structural analysis found that the V3 region of this virus has the characteristic 7T and 25R and a substitution of conserved GPGQ crown motif for GPGH. This study provided compelling evidence that CRF07_BC has the ability to evolve into CXCR4 strains. Our study also lay down the groundwork for studies on tropism switch, which were commonly done for other HIV-1 subtypes, for the long-delayed CRF07_BC.
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