Effect of Spiny-Cheek Crayfish (Faxonius limosus) on H2O2-Induced Oxidative Stress in Normal Fibroblast Cells

Faxonius limosus (spiny-cheek) crayfish is an invasive species that is widespread in Europe. The aim of the study was to evaluate the possibility of using extracts from this crustacean as a source of natural antioxidants. For this purpose, selected bioactive compounds (total phenols, glutathione, vitamins A, E, D, beta-carotene and amino acids) were characterised and the antioxidant activity of the extract was assessed in vitro and in relation to HFFF-2 fibroblast cells, in which oxidative stress was caused by the additive hydrogen peroxide (H2O2). The extract abolished the cytotoxicity of H2O2, decreased reactive oxygen species (ROS) secretion, increased cell viability and decreased the expression of caspase-3. The results suggest that F. limosus extract is a promising raw material with antioxidant properties.

Automated summary

The aim of this study was to evaluate the possibility of using extracts from the spiny-cheek crayfish as natural antioxidants. The results showed that the extract had antioxidant activity and could reduce oxidative stress-induced cell damage.