Upgraded Combined Inject-and-Transfer System for Serial Femtosecond Crystallography

Article Multidisciplinary Sciences

Beef tallow injection matrix for serial crystallography

Ki Hyun Nam

Summary: Serial crystallography allows the visualization of dynamic processes in macromolecular structures at room temperature, and beef tallow is introduced as a stable and controllable sample delivery medium in SX experiments.

SCIENTIFIC REPORTS (2022)

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Article Chemistry, Multidisciplinary

Recent Progress of the PAL-XFEL

Intae Eom et al.

Summary: Since its opening to users in 2017, the X-ray free-electron laser (PAL-XFEL) at the Pohang Accelerator Laboratory has made significant progress in operation and beamline experiments. The PAL-XFEL has achieved increased pulse and photon energy, self-seeding FEL generation, and trials of two-color operation, resulting in excellent scientific results through efficient user experiments.

APPLIED SCIENCES-BASEL (2022)

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Article Biochemistry & Molecular Biology

Transient state measurements on proteins by time-resolved crystallography

Tek Narsingh Malla et al.

Summary: The field of time-resolved crystallography (TRX) has reached new heights with the development of synchrotron light sources. With the advent of X-ray free electron lasers and the support of serial crystallography, TRX can now investigate a wide range of biological systems. This short review provides insights and connections between recent and past results.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2022)

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Article Chemistry, Multidisciplinary

Combination of an inject-and-transfer system for serial femtosecond crystallography

Keondo Lee et al.

Summary: Serial femtosecond crystallography (SFX) is a method that enables the determination of macromolecular crystal structures at room temperature with minimal radiation damage and provides time-resolved molecular dynamics. A combined inject-and-transfer system (BITS) method for sample delivery in SFX experiments has been introduced, allowing reliable deposition of solution samples on UV ozone-treated polyimide films. This method has been successfully applied to determine the room-temperature structure of lysozyme.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2022)

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Article Biochemistry & Molecular Biology

Room-Temperature Structure of Xylitol-Bound Glucose Isomerase by Serial Crystallography: Xylitol Binding in the M1 Site Induces Release of Metal Bound in the M2 Site

Ki Hyun Nam

Summary: The study reveals that xylitol binding to GI causes a change in the geometric coordination of metal binding sites, leading to the rearrangement of metal-binding residues at the M1 and M2 sites. This sheds light on the reason for the decreased metal-binding affinity and enhances understanding of the xylitol-bound state of SruGI for potential industrial applications.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Review Biophysics

Macromolecular movies, storybooks written by nature

Marius Schmidt

Summary: The article summarizes the research progress and recent achievements of the first free electron laser for hard X-rays, highlighting the significant potential of XFELs in time-resolved studies of biological macromolecules.

BIOPHYSICAL REVIEWS (2021)

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Article Multidisciplinary Sciences

An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography

Agata Butryn et al.

Summary: Serial femtosecond crystallography has provided new opportunities for studying protein dynamics with high resolution. However, the challenge lies in studying enzyme-catalyzed reactions. A drop-on-drop sample delivery system has been proposed in this study, showing potential for widespread application in studying enzyme reactions in crystallography.

NATURE COMMUNICATIONS (2021)

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Article Chemistry, Multidisciplinary

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

Suraj Pandey et al.

Summary: This study utilized the high repetition rate of EuXFEL and mix-and-inject technology to observe the initial phase of ceftriaxone binding to Mycobacterium tuberculosis beta-lactamase in real time, demonstrating the importance of this technique for biomedically relevant research.

IUCRJ (2021)

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Article Chemistry, Multidisciplinary

Stable sample delivery in a viscous medium via a polyimide-based single-channel microfluidic chip for serial crystallography

Ki Hyun Nam et al.

Summary: Serial crystallography (SX) allows for room-temperature crystal structures with minimal radiation damage and molecular dynamics comprehension. A stable system for sample delivery in a viscous medium via a polyimide-based single-channel microfluidic (PSM) chip was developed, successfully determining room-temperature lysozyme crystal structures at 1.85 angstrom resolution. Ultimately, this method greatly facilitates sample delivery for SX experiments using synchrotron X-rays.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2021)

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Review Biochemistry & Molecular Biology

Protein X-ray Crystallography and Drug Discovery

Laurent Maveyraud et al.

MOLECULES (2020)

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Article Multidisciplinary Sciences

Shortening injection matrix for serial crystallography

Ki Hyun Nam

SCIENTIFIC REPORTS (2020)

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Article Biochemistry & Molecular Biology

Polysaccharide-Based Injection Matrix for Serial Crystallography

Ki Hyun Nam

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Article Chemistry, Multidisciplinary

3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow

Diana C. F. Monteiro et al.

IUCRJ (2020)

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Article Chemistry, Multidisciplinary

Stable sample delivery in viscous media via a capillary for serial crystallography

Ki Hyun Nam

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2020)

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Article Chemistry, Multidisciplinary

Application of a high-throughout microcrystal delivery system to serial femtosecond crystallography

Donghyeon Lee et al.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2020)

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Article Biotechnology & Applied Microbiology

Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases

Zhengyang Li et al.

BIOTECHNOLOGY FOR BIOFUELS (2020)

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Article Chemistry, Multidisciplinary

Viscous-medium-based crystal support in a sample holder for fixed-target serial femtosecond crystallography

Keondo Lee et al.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2020)

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Article Instruments & Instrumentation

Microfluidic polyimide gas dynamic virtual nozzles for serial crystallography

Mohammad Vakili et al.

REVIEW OF SCIENTIFIC INSTRUMENTS (2020)

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Article Biochemistry & Molecular Biology

Lard Injection Matrix for Serial Crystallography

Ki Hyun Nam

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Article Multidisciplinary Sciences

Segmented flow generator for serial crystallography at the European X-ray free electron laser

Austin Echelmeier et al.

NATURE COMMUNICATIONS (2020)

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Review Biochemistry & Molecular Biology

Sample Delivery Media for Serial Crystallography

Ki Hyun Nam

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

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Article Multidisciplinary Sciences

Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography

Donghyeon Lee et al.

SCIENTIFIC REPORTS (2019)

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Article Instruments & Instrumentation

The serial millisecond crystallography instrument at the Australian Synchrotron incorporating the Lipidico injector

P. Berntsen et al.

REVIEW OF SCIENTIFIC INSTRUMENTS (2019)

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Article Chemistry, Multidisciplinary

1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector

A. Tolstikova et al.

IUCRJ (2019)

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Article Instruments & Instrumentation

Sample delivery using viscous media, a syringe andasyringe pump for serial crystallography

Suk-Youl Park et al.

JOURNAL OF SYNCHROTRON RADIATION (2019)

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Article Multidisciplinary Sciences

Polyacrylamide injection matrix for serial femtosecond crystallography

Jaehyun Park et al.

SCIENTIFIC REPORTS (2019)

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Article Instruments & Instrumentation

Focusing X-ray free-electron laser pulses using Kirkpatrick-Baez mirrors at the NCI hutch of the PAL-XFEL

Jangwoo Kim et al.

JOURNAL OF SYNCHROTRON RADIATION (2018)

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Article Instruments & Instrumentation

Multifarious injection chamber for molecular structure study (MICOSS) system: development and application for serial femtosecond crystallography at Pohang Accelerator Laboratory X-ray Free-Electron Laser

Jaehyun Park et al.

JOURNAL OF SYNCHROTRON RADIATION (2018)

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Article Optics

Hard X-ray free-electron laser with femtosecond-scale timing jitter

Heung-Sik Kang et al.

NATURE PHOTONICS (2017)

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Editorial Material Chemistry, Multidisciplinary

Serial crystallography at synchrotrons and X-ray lasers

Jorg Standfuss et al.

IUCRJ (2017)

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Article Biochemistry & Molecular Biology

Serial femtosecond crystallography: A revolution in structural biology

Jose M. Martin-Garcia et al.

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2016)

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Article Physics, Multidisciplinary

Current status of the CXI beamline at the PAL-XFEL

Jaehyun Park et al.

JOURNAL OF THE KOREAN PHYSICAL SOCIETY (2016)

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Article Chemistry, Multidisciplinary

Recent developments in CrystFEL

Thomas A. White et al.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2016)

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Article Chemistry, Physical

Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

C. Mueller et al.

STRUCTURAL DYNAMICS-US (2015)

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Article Biochemical Research Methods

Grease matrix as a versatile carrier of proteins for serial crystallography

Michihiro Sugahara et al.

NATURE METHODS (2015)

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Article Multidisciplinary Sciences

Goniometer-based femtosecond crystallography with X-ray free electron lasers

Aina E. Cohen et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

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Article Multidisciplinary Sciences

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Uwe Weierstall et al.

NATURE COMMUNICATIONS (2014)

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Article Multidisciplinary Sciences

Fixed-target protein serial microcrystallography with an x-ray free electron laser

Mark S. Hunter et al.

SCIENTIFIC REPORTS (2014)

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Article Chemistry, Multidisciplinary

Room-temperature macromolecular serial crystallography using synchrotron radiation

Francesco Stellato et al.

IUCRJ (2014)

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Article Chemistry, Multidisciplinary

Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data

Anton Barty et al.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2014)

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Article Biochemistry & Molecular Biology

Emerging opportunities in structural biology with X-ray free-electron lasers

Ilme Schlichting et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2012)

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Article Multidisciplinary Sciences

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

Sebastien Boutet et al.

SCIENCE (2012)

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Article Physics, Applied

Gas dynamic virtual nozzle for generation of microscopic droplet streams

D. P. DePonte et al.

JOURNAL OF PHYSICS D-APPLIED PHYSICS (2008)

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Upgraded Combined Inject-and-Transfer System for Serial Femtosecond Crystallography

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