期刊
ADVANCED SCIENCE
卷 9, 期 36, 页码 -出版社
WILEY
DOI: 10.1002/advs.202203631
关键词
gametogenesis; germline stem cells; mitochondrial dynamics; Pld6; progenitor cells
资金
- National Natural Science Foundation of China [32025037, 31721005]
- National R&D Program of China [2018YFA0801000]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010108]
- State Key Laboratory of Freshwater Ecology and Biotechnology [2019FBZ05]
This study identifies zebrafish pld6 as a novel germline-specific gene that plays a crucial role in the maintenance and differentiation of germline stem and progenitor cells (GSPCs). Pld6 knockout mutants develop exclusively into infertile males, as the GSPCs fail to differentiate and undergo apoptosis. Furthermore, pld6-depleted GSPCs display impaired mitochondrial fusion, defects in piRNA biogenesis, and transposon suppression.
Maintenance and differentiation of germline stem and progenitor cells (GSPCs) is important for sexual reproduction. Here, the authors identify zebrafish pld6 as a novel germline-specific gene by cross-analyzing different RNA sequencing results, and find that pld6 knockout mutants develop exclusively into infertile males. In pld6 mutants, GSPCs fail to differentiate and undergo apoptosis, leading to masculinization and infertility. Mitochondrial fusion in pld6-depleted GSPCs is severely impaired, and the mutants exhibit defects in piRNA biogenesis and transposon suppression. Overall, this work uncovers zebrafish Pld6 as a novel germline-specific regulator of mitochondrial fusion, and highlights its essential role in the maintenance and differentiation of GSPCs as well as gonadal development and gametogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据