4.6 Article

Regulatory mechanisms of fatty acids biosynthesis in Armeniaca sibirica seed kernel oil at different developmental stages

期刊

PEERJ
卷 10, 期 -, 页码 -

出版社

PEERJ INC
DOI: 10.7717/peerj.14125

关键词

Armeniaca sibirica; Seed kernel; Fatty acid composition; Biosynthesis; Key enzymes; Transcription factors; Long non-coding RNAs; Regulatory mechanism

资金

  1. National Key Research and Development Program of China [2018YFD1000606-4-1]
  2. Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province

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This study aimed to elucidate the regulatory mechanisms underlying fatty acid biosynthesis in developing seed kernels of Armeniaca sibirica. The researchers identified key enzymes, transcription factors, and long non-coding RNAs involved in fatty acid biosynthesis. This study provides insights for future improvement of seed kernel oil production and quality through breeding.
Background. Armeniaca sibirica seed kernel oil is rich in oleic acid and linoleic acid, thus holding potential value as a source of high-quality edible oils. However, some regulatory factors involved in fatty acids accumulation in A. sibirica seed kernels remain largely elusive. Thus, the aim of this study was to elucidate the regulatory mechanisms underlying fatty acids biosynthesis in A. sibirica developing seed kernels. Methods. Seed kernels from six plants from a single A. sibirica clone were taken at five different developmental stages (days 30, 41, 52, 63, and 73 after anthesis). Fatty acid composition in seed kernel oil was determined by gas chromatography-mass spectrometry (GC-MS). In addition, transcriptome analysis was conducted using second-generation sequencing (SGS) and single-molecule real-time sequencing (SMRT). Results. Rapid accumulation of fatty acids occurred throughout the different stages of seed kernels development, with oleic acid and linoleic acid as the main fatty acids. A total of 10,024, 9,803, 6,004, 6,719 and 9,688 unigenes were matched in the Nt, Nr, KOG, GO and KEGG databases, respectively. In the category lipid metabolism, 228 differentially expressed genes (DEGs) were annotated into 13 KEGG pathways. Specific unigenes encoding 12 key enzymes related to fatty acids biosynthesis were determined. Co-expression network analysis identified 11 transcription factors (TFs) and 13 long non-coding RNAs (lncRNAs) which putatively participate in the regulation of fatty acid biosynthesis. This study provides insights into the molecular regulatory mechanisms of fatty acids biosynthesis in A. sibirica developing seed kernels, and enabled the identification of novel candidate factors for future improvement of the production and quality of seed kernel oil by breeding.

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