期刊
NANOMATERIALS
卷 12, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/nano12193501
关键词
C-60; fullerenes; human serum albumin; folate; photodynamic therapy; phototheranostic platform
类别
资金
- AIRC [22894]
- FIRC-AIRC fellowship, Italy [22318]
- Fondazione Umberto Veronesi, Post-doctoral Fellowships 2022
The study demonstrated the use of human serum albumin (HSA) to disperse fullerenes and build an effective phototheranostic platform by bioconjugating them with folic acid, improving their potential in nanomedicine.
Fullerenes are considered excellent photosensitizers, being highly suitable for photodynamic therapy (PDT). A lack of water solubility and low biocompatibility are, in many instances, still hampering the full exploitation of their potential in nanomedicine. Here, we used human serum albumin (HSA) to disperse fullerenes by binding up to five fullerene cages inside the hydrophobic cavities. Albumin was bioconjugated with folic acid to specifically address the folate receptors that are usually overexpressed in several solid tumors. Concurrently, tetramethylrhodamine isothiocyanate, TRITC, a tag for imaging, was conjugated to C-60@HSA in order to build an effective phototheranostic platform. The in vitro experiments demonstrated that: (i) HSA disperses C-60 molecules in a physiological environment, (ii) HSA, upon C-60 binding, maintains its biological identity and biocompatibility, (iii) the C-60@HSA complex shows a significant visible-light-induced production of reactive oxygen species, and (iv) folate bioconjugation improves both the internalization and the PDT-induced phototoxicity of the C-60@HSA complex in HeLa cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据