期刊
GENES
卷 13, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/genes13091549
关键词
CRISPR-Cas13; bioID; APEX; proximity labeling; RNA elements; RNA-protein interaction
资金
- China Postdoctoral Science Foundation [2021M690694]
This article summarizes the latest advances in CRISPR-guided proximity labeling in studying RNA-protein interactions, and discusses the possibility of applying engineered proximity-labeling enzymes to study RNA-centric interactions in the future.
Proximity labeling employs modified biotin ligases or peroxidases that produce reactive radicals to covalently label proximate proteins with biotin in living cells. The resulting biotinylated proteins can then be isolated and identified. A combination of programmable DNA targeting and proximity labeling that maps proteomic landscape at DNA elements with dCas9-APEX2 has been established in living cells. However, defining interactome at RNA elements has lagged behind. In combination with RNA-targeting CRISPR-Cas13, proximity labeling can also be used to identify proteins that interact with specific RNA elements in living cells. From this viewpoint, we briefly summarize the latest advances in CRISPR-guided proximity labeling in studying RNA-protein interactions, and we propose applying the most recent engineered proximity-labeling enzymes to study RNA-centric interactions in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据