A Tremendous Reorganization Journey for the 3D Chromatin Structure from Gametes to Embryos

The 3D chromatin structure within the nucleus is important for gene expression regulation and correct developmental programs. Recently, the rapid development of low-input chromatin conformation capture technologies has made it possible to study 3D chromatin structures in gametes, zygotes and early embryos in a variety of species, including flies, vertebrates and mammals. There are distinct 3D chromatin structures within the male and female gametes. Following the fertilization of male and female gametes, fertilized eggs undergo drastic epigenetic reprogramming at multi levels, including the 3D chromatin structure, to convert the terminally differentiated gamete state into the totipotent state, which can give rise to an individual. However, to what extent the 3D chromatin structure reorganization is evolutionarily conserved and what the underlying mechanisms are for the tremendous reorganization in early embryos remain elusive. Here, we review the latest findings on the 3D chromatin structure reorganization during embryogenesis, and discuss the convergent and divergent reprogramming patterns and key molecular mechanisms for the 3D chromatin structure reorganization from gametes to embryos in different species. These findings shed light on how the 3D chromatin structure reorganization contribute to embryo development in different species. The findings also indicate the role of the 3D chromatin structure on the acquisition of totipotent developmental potential.

Automated summary

The 3D chromatin structure plays a crucial role in gene expression regulation and embryonic development. Recent advancements in low-input chromatin conformation capture technologies have allowed for the study of 3D chromatin structures in gametes, zygotes, and early embryos in various species. The reorganization of 3D chromatin structure during embryogenesis shows both conserved and divergent patterns across different species, with distinct molecular mechanisms. These findings contribute to our understanding of the contribution of 3D chromatin structure reorganization to embryonic development and its role in acquiring totipotent developmental potential.