期刊
GENES
卷 13, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/genes13101722
关键词
Iso-Seq; lung; bioinformatics; annotation; asthma; horse; Equus caballus
资金
- Swiss National Science Foundation [31003A162548/1]
- Internal Research Fund of the Swiss Institute of Equine Medicine, Bern, Switzerland (ISMEquine Research) [33-890]
The study utilized long-read sequencing technology to generate full-length transcript sequences in equine BALF cells, resulting in 14,234 full-length transcript isoforms. Among these transcripts, 3428 were newly discovered, along with 10,806 known transcripts, including fusion transcripts.
We used Pacific Biosciences long-read isoform sequencing to generate full-length transcript sequences in equine bronchoalveolar lavage fluid (BALF) cells. Our dataset consisted of 313,563 HiFi reads comprising 805 Mb of polished sequence information. The resulting equine BALF transcriptome consisted of 14,234 full-length transcript isoforms originating from 7017 unique genes. These genes consisted of 6880 previously annotated genes and 137 novel genes. We identified 3428 novel transcripts in addition to 10,806 previously known transcripts. These included transcripts absent from existing genome annotations, transcripts mapping to putative novel (unannotated) genes and fusion transcripts incorporating exons from multiple genes. We provide transcript-level data for equine BALF cells as a resource to the scientific community.
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