期刊
FRONTIERS IN PHYSIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2022.1013092
关键词
Plutella xylostella; insect myrosinase; CRISPR/Cas9; plant defense; toxifying gene
类别
资金
- National Natural Science Foundation of China [32172503]
- Natural Science Foundation of Fujian Province [2019J01369]
- 111 program [KRA16001A]
- Science and Technology Foundation of Education Department of Jiangxi Province for Young Scholar [GJJ201450]
- fund of Joint International Laboratory, China [KJG18018A]
This study identified and characterized glycoside hydrolase family 1 members in Plutella xylostella, a specialist pest of crucifers. The study revealed the role of the gene Px008848 in negatively affecting larvae development when feeding on the host plant Arabidopsis thaliana.
Glycoside hydrolase family 1 (GH1) members exhibit a broad substrate spectrum and play important roles in insect-plant interactions, such as the defensive beta-glucosidase and beta-thioglucosidase (so-called myrosinase). However, knowledge about the expression profiling and function of glycoside hydrolase family 1 members in a specialist pest of crucifers Plutella xylostella is still limited. In this study, 13 putative glycoside hydrolase family 1 members of P. xylostella were identified based on the sequence characteristics, while no myrosinase activity was detectable in P. xylostella using gas chromatography-mass spectrometry (GC-MS). Expression profiling of these glycoside hydrolase family 1 members identified the midgut-specific gene Px008848 that is induced by host plant. Further experiments revealed that the in vitro expressed Px008848 protein had beta-glucosidase activity and the survival rate of the larvae feeding on wounded Arabidopsis thaliana leaves declined when leaves were treated with purified Px008848 protein. When CRISPR/Cas9-based homozygous mutant larvae of Px008848 and wild-type larvae were respectively transferred onto the A. thaliana, the larval survival rate of the mutant larvae was significantly higher than that of the wild-type individuals. Our work showed that certain insect glycoside hydrolase family 1 gene may have negative effect on the development of larvae feeding on the host plant, which broadened our understandings on the evolutionary function of this gene family in the insect-plant interaction.
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