期刊
EMERGING MICROBES & INFECTIONS
卷 11, 期 1, 页码 2264-2274出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/22221751.2022.2117093
关键词
Candida parapsilosis; outbreak; candidemia; fluconazole resistance; echinocandin tolerance
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP 2017/022037]
- Science Foundation Ireland [19/FFP/6668]
- NIH [R01AI109025]
- Center for Discovery and Innovation
Patients with severe COVID-19 are at risk of acquiring COVID-19-associated candidemia (CAC), often caused by drug-resistant C. parapsilosis. This study reports the largest outbreak of CAC caused by fluconazole-resistant and echinocandin-tolerant C. parapsilosis. The findings underscore the importance of antifungal stewardship and infection control strategies.
Patients presenting with severe COVID-19 are predisposed to acquire secondary fungal infections such as COVID-19-associated candidemia (CAC), which are associated with poor clinical outcomes despite antifungal treatment. The extreme burden imposed on clinical facilities during the COVID-19 pandemic has provided a permissive environment for the emergence of clonal outbreaks of multiple Candida species, including C. auris and C. parapsilosis. Here we report the largest clonal CAC outbreak to date caused by fluconazole resistant (FLZR) and echinocandin tolerant (ECT) C. parapsilosis. Sixty C. parapsilosis strains were obtained from 57 patients at a tertiary care hospital in Brazil, 90% of them were FLZR and ECT. Although only 35.8% of FLZR isolates contained an ERG11 mutation, all of them contained the TAC1(L518F) mutation and significantly overexpressed CDR1. Introduction of TAC1(L518F) into a susceptible background increased the MIC of fluconazole and voriconazole 8-fold and resulted in significant basal overexpression of CDR1. Additionally, FLZR isolates exclusively harboured E1939G outside of Fks1 hotspot-2, which did not confer echinocandin resistance, but significantly increased ECT. Multilocus microsatellite typing showed that 51/60 (85%) of the FLZR isolates belonged to the same cluster, while the susceptible isolates each represented a distinct lineage. Finally, biofilm production in FLZR isolates was significantly lower than in susceptible counterparts Suggesting that it may not be an outbreak determinant. In summary, we show that TAC1(L518F) and FKS1(E1393G) confer FLZR and ECT, respectively, in CAC-associated C. parapsilosis. Our study underscores the importance of antifungal stewardship and effective infection control strategies to mitigate clonal C. parapsilosis outbreaks.
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