4.5 Article

Effect of lycopene as an adjuvant therapy with 5-florouracil in human colon cancer

期刊

出版社

ELSEVIER
DOI: 10.1016/j.sjbs.2022.103392

关键词

Cancer; Colon; Lycopene; 5-Fluorouracil; Cytotoxicity; Apoptosis

类别

资金

  1. King Saud University, Riyadh, Saudi Arabia
  2. [RSP-2021/26]

向作者/读者索取更多资源

Colon cancer is a common malignancy and there is a need for improving the quality of life for patients. This study explored the role of lycopene as an adjuvant therapy with 5-fluorouracil (5-FU) in colon cancer cells. The findings showed that lycopene enhanced apoptosis, suppressed cell migration, and reduced cytotoxicity when combined with 5-FU treatment.
Colon cancer (CC) is among the most frequent human cancers. Although, there is improvement in diag-nostic techniques and existing treatment possibilities. Still, there is an unmet need for a novel treatment regimen that will improve the patient's quality of life. Here, the role of lycopene as an adjuvant therapy with 5-fluorouracil (5-FU) was explored in Caco2 colon cancer cells. Cells were exposed to a dose (3 lg/ ml) of 5-FU and three doses (60, 90, 120 lg/ml) of lycopene either alone or as a mixture with 5-FU. Cytotoxicity, genotoxicity, oxidative stress, gene expression, and apoptotic parameters were investigated in this study. Findings showed that 5-FU or lycopene alone induced a dose-dependent increase in cyto-toxicity which was slightly reduced in lycopene mixtures. Apoptotic assays showed that 5-FU induced a significant level of apoptosis but not necrosis. However, a lycopene mixture with 5-FU enhanced 5-FU triggered apoptosis and promoted necrosis. The mixtures were also shown to suppress mitochondrial membrane potential while gene expression analyses showed the induction of Bax expression upon expo-sure to mix 90 exhibited the highest Bax to Bcl-2 ratio and caspase 3 and 9 gene expression. Furthermore, the mixture treatment also inhibited cell migration in the wound healing assay compared to 5-FU alone. In conclusion, lycopene was found to sensitize Caco 2 cell lines to 5-FU treatment by inducing the expres-sion of apoptotic genes. This, coupled with lycopene suppression of cytotoxicity and cell migration, indi-cates lycopene may be a promising candidate for adjuvant therapy involving 5-FU in CC.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据