4.7 Article

Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar

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FRONTIERS IN PLANT SCIENCE
卷 13, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2022.973305

关键词

poplar; salicylic acid methyltransferase; salicylic acid; methyl salicylate; pathogen resistance

资金

  1. National Key Research and Development Program of China
  2. [2017YFD0600102]
  3. [5101049170175]

向作者/读者索取更多资源

In this study, the function of SAMT in poplar plants was investigated, and it was found to play a crucial role in the defense mechanism against pathogens. Overexpression of SAMT increased the content of MeSA and decreased the content of SA. Additionally, SAMT overexpression led to higher expression of pathogenesis-related genes, but did not confer resistance against the fungal pathogen.
Salicylic acid (SA) is generally considered to be a critical signal transduction factor in plant defenses against pathogens. It could be converted to methyl salicylate (MeSA) for remote signals by salicylic acid methyltransferase (SAMT) and converted back to SA by SA-binding protein 2 (SABP2). In order to verify the function of SAMT in poplar plants, we isolated the full-length cDNA sequence of PagSAMT from 84K poplar and cultivated PagSAMT overexpression lines (OE-2 isolate) to test its role in SA-mediated defenses against the virulent fungal pathogen Botryosphaeria dothidea. Our results showed that after inoculation with B. dothidea, OE-2 significantly increased MeSA content and reduced SA content which is associated with increased expression of SAMT in both infected and uninfected leaves, when compared against the wild type (WT). Additionally, SAMT overexpression plant lines (OE-2) exhibited higher expression of pathogenesis-related genes PR-1 and PR-5, but were still susceptible to B. dothidea suggesting that in poplar SA might be responsible for resistance against this pathogen. This study expands the current understanding of joint regulation of SAMT and SABP2 and the balance between SA and MeSA in poplar responses to pathogen invasion.

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