期刊
FRONTIERS IN MICROBIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.926724
关键词
HTS; mechanical inoculation; Pueraria lobata; Emaravirus; mixed infection
类别
资金
- National Natural Science Foundation of China [SWU-XDPY22002]
- Innovation Research 2035 Pilot Plan of Southwest University [B18044]
- Overseas Expertise Introduction Center for Discipline Innovation (111 Project) [CYS21141]
- Chongqing Postgraduate Research Innovation Project
- [32072389]
Pueraria lobata plants showing symptoms of mosaic, yellow spots, and mottling were found to be infected by a mixed infection of three viruses: Pueraria lobata-associated emaravirus (PloAEV), Pueraria lobata-associated crinivirus (PloACV), and kudzu mosaic virus (KuMV-CQ). The study identified and characterized the genetic makeup of these viruses, and found a high association between them in symptomatic Pueraria lobata plants.
Pueraria lobata (Willd) (Pueraria montana var. lobata (Willd.) Maesen & S. M. Almeida ex Sanjappa & Predeep) is an important herbal medicine used in many countries. In P. lobata plants showing symptoms of mosaic, yellow spots, and mottling, mixed infection of new viruses provisionally named Pueraria lobata-associated emaravirus (PloAEV, genus Emaravirus), Pueraria lobata-associated crinivirus (PloACV, genus Crinivirus), and isolate CQ of the previously reported kudzu mosaic virus (KuMV-CQ, genus Begomovirus) was confirmed through high-throughput sequencing. PloAEV has five RNA segments, encoding a putative RNA-dependent RNA polymerase, glycoprotein precursor, nucleocapsid protein, movement protein, and P5, respectively. PloACV has two RNA segments, encoding 11 putative proteins. Only PloAEV could be mechanically transmitted from mixed infected symptomatic kudzu to Nicotiana benthamiana plants. All three viruses were detected in 35 symptomatic samples collected from five different growing areas, whereas no viruses were detected in 21 non-symptomatic plants, suggesting a high association between these three viruses. Thus, this study provides new knowledge on the diversity and molecular characteristics of viruses in P. lobata plants affected by the viral disease.
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