期刊
FRONTIERS IN MICROBIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.1031388
关键词
Trichophyton; LAMP; microfluidics; early diagnosis; chip
类别
资金
- Hebei Provincial Department of Education [B20221018]
- Science and Technology Bureau of Huzhou, Zhejiang Province [2021GYB01]
In this study, LAMP primers for Trichophyton were designed and a LAMP-Microfluidic chip detection system was developed. The system exhibited high specificity and sensitivity, enabling the detection of six common species of Trichophyton without cross-reactivity with other dermatophytes. The system has the potential for clinical application, offering a simple and efficient way to detect Trichophyton infections.
Trichophyton is the most pathogenic type of fungal skin infection. It often invades and grows in a keratin-rich matrix, and lesions include human skin, hair, and fingernails (toenails). We designed LAMP primers for Trichophyton and developed a LAMP-Microfluidic chip detection system for Trichophyton. This system detects six common species of Trichophyton in the genus Trichophyton, including Trichophyton rubrum, Trichophyton mentagrophyte, Trichophyton violaceum, Trichophyton tonsurans, Trichophyton verrucosum, and Trichophyton schoenleinii. The specificity reached 100%, and the sensitivity could reach about 1 x 10(2) copies/mu l. The entire detection process can be completed within 60 min and does not cross-react with other dermatophytes. The established LAMP-Microfluidic chip detection system has the advantages of simple operation, high specificity, and high sensitivity, and has the potential for clinical application.
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