Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity

Tellurite [Te(IV)] is a high-toxicity metalloid. In this study, a fungus with high Te(IV) resistance was isolated. Strain AB1 could efficiently reduce highly toxic Te(IV) to less toxic Te(0). The reduced products formed rod-shaped biogenetic Te(0) nanoparticles (Bio-TeNPs) intracellularly. Further TEM-element mapping, FTIR, and XPS analysis showed that the extracted Bio-TeNPs ranged from 100 to 500 nm and consisted of Te(0), proteins, lipids, aromatic compounds, and carbohydrates. Moreover, Bio-TeNPs exhibited excellent antibacterial ability against Shigella dysenteriae, Escherichia coli, Enterobacter sakazakii, and Salmonella typhimurium according to inhibition zone tests. Further growth and live/dead staining experiments showed that E. coli and S. typhimurium were significantly inhibited by Bio-TeNPs, and cells were broken or shriveled after treatment with Bio-TeNPs based on SEM observation. Additionally, the antioxidant and cytotoxicity tests showed that the Bio-TeNPs exhibited excellent antioxidant capacity with no cytotoxicity. All these results suggested that strain AB1 showed great potential in bioremediation and Bio-TeNPs were excellent antibacterial nanomaterials with no cytotoxicity.

Automated summary

In this study, a fungus with high resistance to toxic tellurite [Te(IV)] was isolated. The fungus efficiently reduced Te(IV) to less toxic Te(0), forming rod-shaped biogenic nanoparticles (Bio-TeNPs) intracellularly. The extracted Bio-TeNPs consisted of Te(0), proteins, lipids, aromatic compounds, and carbohydrates, and exhibited excellent antibacterial ability against various bacteria. Moreover, the Bio-TeNPs showed excellent antioxidant capacity and no cytotoxicity. These findings suggest the potential of strain AB1 in bioremediation and the Bio-TeNPs as antibacterial nanomaterials.