期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2022.991849
关键词
Enterococcus faecalis; recombinase polymerase amplification; lateral flow strip; molecular detection; qPCR
资金
- Zhenjiang Key R&D Program (Social Development) [SSH20220140238]
- Scientific research project of Bengbu Medical College [BYKY2019253ZD]
- Jiangsu University 2021 Clinical Medicine Science and Technology Development Fund (Natural Science Category) [JLY2021087]
- Lianyungang Second People's Hospital 2020 Hospital Young and Middle-aged Medical Talent Growth Fund [TQ202006]
- Guangxi innovation-driven development project [2021YFE0111100]
- National Key R&D Program of China [GJ2021015]
- Zhenjiang Science and Technology [CARS-18]
- the earmarked fund [AA19182012-2]
Enterococcus faecalis is a serious issue in hospitals, necessitating the development of rapid and accurate detection methods. In this study, a visualized isothermal amplification method using RPA-LFS was developed for E. faecalis detection, showing fast, accurate, specific, and compatible results.
Enterococcus faecalis is a serious problem for hospitals and can spread from patient to patient. Most of the current detection methods are associated with limitations associated with the need for trained personnel; they are also time-consuming. Thus, it is necessary to develop rapid and accurate detection methods to control the spread of E. faecalis. In this study, we developed a rapid and accurate detection method for E. faecalis using recombinase polymerase amplification (RPA) combined with a lateral flow strip (LFS). This method could be completed in approximately 35 min at 37 degrees C. The limit of detection was 10 CFU/mu L, irrespective of whether the templates were pure or complex. This method also showed good specificity and compatibility. In total, 278 clinical samples were tested using the RPA-LFS method; the detection accuracy was equal to that of the conventional qPCR method. This visualized isothermal amplification method could be useful for the future on-site detection of E. faecalis.
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