4.6 Article

Substrate-Dependent Cellulose Saccharification Efficiency and LPMO Activity of Cellic CTec2 and a Cellulolytic Secretome from Thermoascus aurantiacus and the Impact of H2O2-Producing Glucose Oxidase

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Agricultural Engineering

Improving cellulases hydrolytic action: An expanded role for electron donors of lytic polysaccharide monooxygenases in cellulose saccharification

Donglin Xin et al.

Summary: This study evaluated the effects of ascorbic acid and gallic acid on cellulase hydrolytic action and found that ascorbic acid increased the hydrolysis of cellulose by cellulases, while gallic acid had no effect. The effect of ascorbic acid differed for different types of cellulase enzymes.

BIORESOURCE TECHNOLOGY (2022)

Article Biochemistry & Molecular Biology

The impact of reductants on the catalytic efficiency of a lytic polysaccharide monooxygenase and the special role of dehydroascorbic acid

Anton A. Stepnov et al.

Summary: A novel LPMO, SscLPMO10B from Streptomyces scabies, shows a clear correlation between its catalytic rate and H2O2 generation rate in the reaction mixture. The impact of oxidised ascorbic acid on LPMO activity suggests that apparent monooxygenase activity in SscLPMO10B reactions is actually a peroxygenase reaction.

FEBS LETTERS (2022)

Article Biochemical Research Methods

Chromatographic analysis of oxidized cello-oligomers generated by lytic polysaccharide monooxygenases using dual electrolytic eluent generation

Heidi Ostby et al.

Summary: Research on oligosaccharides, including the product mixtures generated by LPMOs, is growing rapidly. A novel method based on dual electrolytic eluent generation has been developed to separate and quantify these compounds with high sensitivity and improved analytical precision. This new chromatographic platform offers simplified operation and rapid method optimization, overcoming drawbacks associated with manual eluent preparation.

JOURNAL OF CHROMATOGRAPHY A (2022)

Article Chemistry, Multidisciplinary

2-Naphthol Impregnation Prior to Steam Explosion Promotes LPMO-Assisted Enzymatic Saccharification of Spruce and Yields High-Purity Lignin

Line Degn Hansen et al.

Summary: The use of a carbocation scavenger to improve the enzymatic saccharification of steam-exploded softwood brings us closer to a softwood-based biorefinery. The impregnation effect enables complete saccharification of spruce cellulose with lytic polysaccharide monooxygenase (LPMO)-containing Cellic CTec2, but not with an LPMO-poor cellulase cocktail (Celluclast).

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2022)

Article Multidisciplinary Sciences

Enhanced in situ H2O2 production explains synergy between an LPMO with a cellulose-binding domain and a single-domain LPMO

Anton A. Stepnov et al.

Summary: Lytic polysaccharide monooxygenases (LPMOs) are mono-copper enzymes that catalyze oxidative depolymerization of recalcitrant substrates such as chitin or cellulose. Recent research has shown that LPMOs can catalyze fast peroxygenase reactions and that the presence of a cellulose-binding module (CBM) can affect the in situ production of H2O2. This study investigated the interplay between a CBM-containing LPMO variant and a truncated form without the CBM. The results demonstrate that truncation of the CBM leads to increased H2O2 production and decreased enzyme stability. Furthermore, the combination of the two enzyme forms results in synergistic effects, improving cellulose degradation while reducing enzyme inactivation caused by off-pathway reactions with excess H2O2.

SCIENTIFIC REPORTS (2022)

Article Biotechnology & Applied Microbiology

Unraveling the roles of the reductant and free copper ions in LPMO kinetics

Anton A. Stepnov et al.

Summary: The study describes a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, and investigates the effects of the reductant and copper ions on the LPMO reaction. Results show that ascorbate-driven LPMO reactions are extremely sensitive to very low amounts of free copper, whereas reactions driven by gallic acid are almost unaffected by the presence of free copper ions.

BIOTECHNOLOGY FOR BIOFUELS (2021)

Article Biochemistry & Molecular Biology

Fast and Specific Peroxygenase Reactions Catalyzed by Fungal Mono-Copper Enzymes

Lukas Rieder et al.

Summary: The study reveals that LPMOs can efficiently perform peroxygenase activity and have higher oxidase activity compared to monooxygenases, which is attributed to the unique arrangement of their catalytic sites.

BIOCHEMISTRY (2021)

Article Biochemistry & Molecular Biology

Insights into the H2O2-driven catalytic mechanism of fungal lytic polysaccharide monooxygenases

Tobias M. Hedison et al.

Summary: Understanding the reaction mechanism of fungal LPMOs with H2O2 is crucial for their application in biotechnological settings. Research shows that a 'priming' electron transfer reaction from the cellobiose dehydrogenase partner protein supports up to 20 catalytic cycles of LPMOs.

FEBS JOURNAL (2021)

Article Biochemistry & Molecular Biology

Kinetics of H2O2-driven catalysis by a lytic polysaccharide monooxygenase from the fungus Trichoderma reesei

Silja Kuusk et al.

Summary: This study provides kinetic characterization of the cellulose-degrading model fungus Trichoderma reesei's LPMO TrAA9A, revealing that the catalytic efficiency of cellulose peroxygenase reaction is significantly higher than that of the reductant peroxidase reaction. It is suggested that the ping-pong mechanism in the ascorbic acid peroxidase reaction leads to irreversible inactivation of the enzyme with a certain probability. Theoretical analysis proposes a relationship between the LPMO half-life, kinetic parameters, and reactant concentrations.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Biotechnology & Applied Microbiology

In situ measurements of oxidation-reduction potential and hydrogen peroxide concentration as tools for revealing LPMO inactivation during enzymatic saccharification of cellulose

Adnan Kadic et al.

Summary: Biochemical conversion of lignocellulosic biomass to sugars is hindered by high saccharifying enzyme costs. LPMOs offer potential solutions, but overdosing H2O2 can lead to enzyme inactivation. In this study, real-time monitoring of oxidation-reduction potential and H2O2 concentration allowed for predicting LPMO inactivation during enzymatic saccharification, potentially improving overall yields.

BIOTECHNOLOGY FOR BIOFUELS (2021)

Article Biochemistry & Molecular Biology

A comparative biochemical investigation of the impeding effect of C1-oxidizing LPMOs on cellobiohydrolases

Malene Billeskov Keller et al.

Summary: The study found that C1-oxidizing LPMOs have varying effects on the activity of different reducing-end cellobiohydrolases, with oxidation reducing enzyme activity and substrate complexation rate. This finding suggests that C1-oxidized chain ends are not ideal sites for attack by reducing-end cellobiohydrolases, which could be relevant for optimizing industrial enzyme cocktails.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Chemistry, Multidisciplinary

H2O2 in Liquid Fractions of Hydrothermally Pretreated Biomasses: Implications of Lytic Polysaccharide Monooxygenases

Riin Kont et al.

Summary: This study evaluated the dynamics of H2O2 in the liquid fractions of different biomasses using an LPMO-based detection tool, revealing that H2O2 acts as an intermediate in the oxidation of compounds in the liquid fractions, with its formation rate higher than its accumulation rate. The liquid fractions of different biomasses have varying effects on the formation of H2O2 and the enzymatic degradation of cellulose.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

Article Biotechnology & Applied Microbiology

Demonstration-scale enzymatic saccharification of sulfite-pulped spruce with addition of hydrogen peroxide for LPMO activation

Thales H. F. Costa et al.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR (2020)

Article Biotechnology & Applied Microbiology

Purification and characterization of a native lytic polysaccharide monooxygenase fromThermoascus aurantiacus

Susanne Fritsche et al.

BIOTECHNOLOGY LETTERS (2020)

Article Chemistry, Physical

Activation of O2 and H2O2 by Lytic Polysaccharide Monooxygenases

Binju Wang et al.

ACS CATALYSIS (2020)

Review Biotechnology & Applied Microbiology

On the functional characterization of lytic polysaccharide monooxygenases (LPMOs)

Vincent G. H. Eijsink et al.

BIOTECHNOLOGY FOR BIOFUELS (2019)

Review Biotechnology & Applied Microbiology

A review on commercial-scale high-value products that can be produced alongside cellulosic ethanol

Oscar Rosales-Calderon et al.

BIOTECHNOLOGY FOR BIOFUELS (2019)

Review Biotechnology & Applied Microbiology

Pretreatment for biorefineries: a review of common methods for efficient utilisation of lignocellulosic materials

Mats Galbe et al.

BIOTECHNOLOGY FOR BIOFUELS (2019)

Article Biochemistry & Molecular Biology

Kinetics of H2O2-driven degradation of chitin by a bacterial lytic polysaccharide monooxygenase

Silja Kuusk et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2018)

Article Biotechnology & Applied Microbiology

Laccase-derived lignin compounds boost cellulose oxidative enzymes AA9

Livia Brenelli et al.

BIOTECHNOLOGY FOR BIOFUELS (2018)

Article Biotechnology & Applied Microbiology

Redox processes acidify and decarboxylate steam-pretreated lignocellulosic biomass and are modulated by LPMO and catalase

Ausra Peciulyte et al.

BIOTECHNOLOGY FOR BIOFUELS (2018)

Article Multidisciplinary Sciences

Reactivity of O-2 versus H2O2 with polysaccharide monooxygenases

John A. Hangasky et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Article Biotechnology & Applied Microbiology

The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

Gerdt Mueller et al.

BIOTECHNOLOGY FOR BIOFUELS (2018)

Article Biotechnology & Applied Microbiology

Effect of lignin fractions isolated from different biomass sources on cellulose oxidation by fungal lytic polysaccharide monooxygenases

Madhu Nair Muraleedharan et al.

BIOTECHNOLOGY FOR BIOFUELS (2018)

Article Biotechnology & Applied Microbiology

Development of minimal enzyme cocktails for hydrolysis of sulfite-pulped lignocellulosic biomass

Piotr Chylenski et al.

JOURNAL OF BIOTECHNOLOGY (2017)

Article Biochemistry & Molecular Biology

Oxidative cleavage of polysaccharides by monocopper enzymes depends on H2O2

Bastien Bissaro et al.

NATURE CHEMICAL BIOLOGY (2017)

Article Chemistry, Multidisciplinary

Oxygen Transfer in High Solids Loading and Highly Viscous Lignocellulose Hydrolysates

Weiliang Hou et al.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2017)

Article Biotechnology & Applied Microbiology

Xylose induces cellulase production in Thermoascus aurantiacus

Timo Schuerg et al.

BIOTECHNOLOGY FOR BIOFUELS (2017)

Article Biotechnology & Applied Microbiology

Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs

Piotr Chylenski et al.

BIOTECHNOLOGY FOR BIOFUELS (2017)

Article Multidisciplinary Sciences

Extracellular electron transfer systems fuel cellulose oxidative degradation

Daniel Kracher et al.

SCIENCE (2016)

Article Biotechnology & Applied Microbiology

Harnessing the potential of LPMO-containing cellulase cocktails poses new demands on processing conditions

Gerdt Muller et al.

BIOTECHNOLOGY FOR BIOFUELS (2015)

Article Chemistry, Multidisciplinary

Lignocellulose pretreatment technologies affect the level of enzymatic cellulose oxidation by LPMO

Ursula Fabiola Rodriguez-Zuniga et al.

GREEN CHEMISTRY (2015)

Article Multidisciplinary Sciences

Enzymatic cellulose oxidation is linked to lignin by long-range electron transfer

Bjorge Westereng et al.

SCIENTIFIC REPORTS (2015)

Review Biochemistry & Molecular Biology

New enzyme insights drive advances in commercial ethanol production

Paul V. Harris et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2014)

Review Biotechnology & Applied Microbiology

Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels

Venkatesh Balan et al.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR (2013)

Article Agricultural Engineering

History and future of world's most advanced biorefinery in operation

Gudbrand Rodsrud et al.

BIOMASS & BIOENERGY (2012)

Article Biotechnology & Applied Microbiology

Production and effect of aldonic acids during enzymatic hydrolysis of lignocellulose at high dry matter content

David Cannella et al.

BIOTECHNOLOGY FOR BIOFUELS (2012)

Article Biotechnology & Applied Microbiology

Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay

Roman Kittl et al.

BIOTECHNOLOGY FOR BIOFUELS (2012)

Article Biotechnology & Applied Microbiology

Thermoascus aurantiacus is a promising source of enzymes for biomass deconstruction under thermophilic conditions

Shara D. McClendon et al.

BIOTECHNOLOGY FOR BIOFUELS (2012)

Review Biotechnology & Applied Microbiology

Novel enzymes for the degradation of cellulose

Svein Jarle Horn et al.

BIOTECHNOLOGY FOR BIOFUELS (2012)

Article Chemistry, Multidisciplinary

Oxidative Cleavage of Cellulose by Fungal Copper-Dependent Polysaccharide Monooxygenases

William T. Beeson et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)

Review Microbiology

Trichoderma reesei RUT-C30-thirty years of strain improvement

Robyn Peterson et al.

MICROBIOLOGY-SGM (2012)

Article Biochemistry & Molecular Biology

Cellobiose Dehydrogenase and a Copper-Dependent Polysaccharide Monooxygenase Potentiate Cellulose Degradation by Neurospora crassa

Christopher M. Phillips et al.

ACS CHEMICAL BIOLOGY (2011)

Article Biotechnology & Applied Microbiology

Deactivation of cellulases by phenols

Eduardo Ximenes et al.

ENZYME AND MICROBIAL TECHNOLOGY (2011)

Article Multidisciplinary Sciences

Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components

R. Jason Quinlan et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)

Article Multidisciplinary Sciences

An Oxidative Enzyme Boosting the Enzymatic Conversion of Recalcitrant Polysaccharides

Gustav Vaaje-Kolstad et al.

SCIENCE (2010)

Review Biotechnology & Applied Microbiology

Enzymatic conversion of lignocellulose into fermentable sugars: challenges and opportunities

Henning Jorgensen et al.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR (2007)

Article Biotechnology & Applied Microbiology

Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations

Alex Berlin et al.

JOURNAL OF BIOTECHNOLOGY (2006)

Article Biochemistry & Molecular Biology

Thermal inactivation of glucose oxidase - Mechanism and stabilization using additives

MD Gouda et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2003)

Review Biotechnology & Applied Microbiology

Regulation of Aspergillus genes encoding plant cell wall polysaccharide-degrading enzymes;: relevance for industrial production

RP de Vries

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2003)