4.6 Article

Production of Putrescine in Metabolic Engineering Corynebacterium crenatum by Mixed Sugar Fermentation

期刊

ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 10, 期 44, 页码 14407-14416

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.2c02602

关键词

Corynebacterium crenatum; putrescine; metabolic engineering; ribosome binding site; xylose

资金

  1. National Key Research and Development Program of China [2021YFC2100900]
  2. National Natural Science Foundation of China [32070035]
  3. Key Research and Development Program of the Ningxia Hui Autonomous Region [2019BCH01002]
  4. Fundamental Research Funds for the Central Universities [JUSRP221012]
  5. 111 Project [111-2-06]

向作者/读者索取更多资源

In this study, metabolic engineering strategies were designed to significantly enhance putrescine production in Corynebacterium crenatum SYPA. By optimizing the synthetic pathway, regulating gene expression, blocking degradation pathways, and constructing a xylose utilization pathway, the titer of putrescine was greatly increased. The engineered strains also showed the potential to produce putrescine from biomass hydrolysates.
Putrescine, a biogenic diamine, serves as an important component in various polyamides, medicine, and surfactants. One challenge for efficient putrescine production is to construct high-efficiency microbial cells. In this study, we designed the metabolic engineering strategies to significantly enhance the putrescine titer in Corynebacterium crenatum SYPA. First, an optimal synthetic pathway of putrescine comprising arginine decarboxylase (speA) and agmatinase (speB) genes from Escherichia coli was selected and introduced into C. crenatum. For efficient production of putrescine, the expression of agmatinase was optimized through a ribosome binding site regulation strategy. Next, the putrescine yield was furthermore increased by the knockout of snaA, speE, and cgmR to block putrescine degradation and overexpression of exporter CgmA. Additionally, the xylose utilization pathway was constructed for putrescine synthesis. Finally, the titer of putrescine was 41.5, 36.8, and 33.4 g/L from glucose, mixed sugar, and simulated wheat straw hydrolysates by fed-batch culture for 72 h, respectively. The yield was 0.18 g/g glucose, 0.15 g/g sugar, and 0.14 g/g sugar, respectively. To our knowledge, those results were the highest putrescine titers ever reported in the engineering of C. crenatum and Corynebacterium glutamicum. The engineering strains had the potential to produce putrescine from biomass hydrolysates.

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