期刊
TOXINS
卷 14, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/toxins14100702
关键词
zearalenone; weaned piglets; intestine; histology; intestinal permeability; proteomic
资金
- Shandong science and technology-based small and medium-sized enterprises innovation capacity improvement project [2022TSGC1275]
- Natural Science Foundation of Shandong Province [ZR2021MC048]
- Shandong province pig industry technology system [SDAIT-08-04]
This experiment investigated the changes in the proteomics of ZEN-induced intestinal damage in weaned piglets. The results showed that ZEN exposure caused intestinal damage and altered the expression of important proteins.
Zearalenone (ZEN), also known as the F-2 toxin, is a common contaminant in cereal crops and livestock products. This experiment aimed to reveal the changes in the proteomics of ZEN-induced intestinal damage in weaned piglets by tandem mass spectrometry tags. Sixteen weaned piglets either received a basal diet or a basal diet supplemented with 3.0 mg/kg ZEN in a 32 d study. The results showed that the serum levels of ZEN, alpha-zearalenol, and beta-zearalenol were increased in weaned piglets exposed to ZEN (p < 0.05). Zearalenone exposure reduced apparent nutrient digestibility, increased intestinal permeability, and caused intestinal damage in weaned piglets. Meanwhile, a total of 174 differential proteins (DEPs) were identified between control and ZEN groups, with 60 up-regulated DEPs and 114 down-regulated DEPs (FC > 1.20 or <0.83, p < 0.05). Gene ontology analysis revealed that DEPs were mainly involved in substance transport and metabolism, gene expression, inflammatory, and oxidative stress. The Kyoto Encyclopedia of Genes and Genomes analysis revealed that DEPs were significantly enriched in 25 signaling pathways (p < 0.05), most of which were related to inflammation and amino acid metabolism. Our study provides valuable clues to elucidate the possible mechanism of ZEN-induced intestinal injury.
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