期刊
PROTEIN & CELL
卷 14, 期 4, 页码 279-293出版社
OXFORD UNIV PRESS
DOI: 10.1093/procel/pwac038
关键词
single-nucleus RNA-sequencing; primate; aging; FOXP1; cardiomyocyte
类别
Aging is a major risk factor for cardiovascular diseases, but the specific changes in cell types during cardiac aging are not clear. Through single-nucleus RNA sequencing analysis, we found that aged cardiomyocytes had a significant decrease in cell numbers and fluctuations in transcriptional profiles. We identified FOXP1 as a key downregulated factor in aged cardiomyocytes, and its dysregulation was associated with heart function and cardiac diseases. Our findings provide insights into the cellular and molecular changes in ventricular aging and potential targets for intervention.
Aging poses a major risk factor for cardiovascular diseases, the leading cause of death in the aged population. However, the cell type-specific changes underlying cardiac aging are far from being clear. Here, we performed single-nucleus RNA-sequencing analysis of left ventricles from young and aged cynomolgus monkeys to define cell composition changes and transcriptomic alterations across different cell types associated with age. We found that aged cardiomyocytes underwent a dramatic loss in cell numbers and profound fluctuations in transcriptional profiles. Via transcription regulatory network analysis, we identified FOXP1, a core transcription factor in organ development, as a key downregulated factor in aged cardiomyocytes, concomitant with the dysregulation of FOXP1 target genes associated with heart function and cardiac diseases. Consistently, the deficiency of FOXP1 led to hypertrophic and senescent phenotypes in human embryonic stem cell-derived cardiomyocytes. Altogether, our findings depict the cellular and molecular landscape of ventricular aging at the single-cell resolution, and identify drivers for primate cardiac aging and potential targets for intervention against cardiac aging and associated diseases.
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