期刊
ADVANCED HEALTHCARE MATERIALS
卷 11, 期 18, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202201679
关键词
bilayer struvite-nanowire-doped membranes; craniomaxillofacial bone defects; critical-sized calvarial defect repair; guided bone regeneration; osteoconductive struvite nanowires
资金
- Hong Kong Special Administration Region (HKSAR) Collaborative Research Fund [CRF C4026-17 W]
- Areas of Excellence [AoE/M-402/20]
- National Natural Science Foundation of China [82071127]
- Med-X Innovation Programme of Mex-X Center for Materials [MCM202106]
- Research and Develop Programme [RD-03-202105]
Guided bone regeneration (GBR) therapy has shown significant curative effects on craniomaxillofacial (CMF) bone defects. A bilayer struvite-doped membrane was developed for GBR treatment, which exhibited a well-developed heterogeneous architecture, satisfactory mechanical performance, and long-lasting characteristics. In vitro evaluations revealed that the membrane not only acted as a strong barrier but also actively promoted cellular adhesion, proliferation, and osteogenic differentiation. In vivo experiments demonstrated that the struvite-doped membrane significantly improved the healing outcomes of critical-sized calvarial defects in rats, leading to enhanced osteogenesis and new bone formation. These promising preclinical results suggest the great potential of the bilayer struvite-doped membrane as a GBR device for large-area CMF bone reconstruction.
Guided bone regeneration (GBR) therapy demonstrates a prominent curative effect on the management of craniomaxillofacial (CMF) bone defects. In this study, a GBR membrane consisting of a microporous layer and a struvite-nanowire-doped fibrous layer is constructed via non-solvent induced phase separation, followed by an electrospinning procedure to treat critical-sized calvarial defects. The microporous layer shows selective permeability for excluding the rapid-growing non-osteogenic tissues and potential wound stabilization. The nanowire-like struvite is synthesized as the deliverable therapeutic agent within the fibrous layer to facilitate bone regeneration. Such a membrane displays a well-developed heterogeneous architecture, satisfactory mechanical performance, and long-lasting characteristics. The in vitro biological evaluation reveals that apart from being a strong barrier, the bilayer struvite-laden membrane can actively promote cellular adhesion, proliferation, and osteogenic differentiation. Consequently, the multifunctional struvite-doped membranes are applied to treat 5 mm-sized bilateral calvarial defects in rats, resulting in overall improved healing outcomes compared with the untreated or the struvite-free membrane-treated group, which is characterized by enhanced osteogenesis and significantly increased new bone formation. The encouraging preclinical results reveal the great potential of the bilayer struvite-doped membrane as a clinical GBR device for augmenting large-area CMF bone reconstruction.
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