期刊
ACS SYNTHETIC BIOLOGY
卷 11, 期 11, 页码 3583-3591出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.2c00291
关键词
synthetic biology; DNA storage; Bacillus spore; artificial chromosome
资金
- Ministry of Science and Technology, National Key RD Program [2018YF0902604]
- National Natural Science Foundation of China?s Research Fund for International Young Scientists [22050410270]
- Shenzhen Special Fund for Innovation and Entrepreneurship of Overseas High-level Talents Peacock Team [KQTD20170810111314625]
- Guangdong Innovative and Entrepreneurial Research Team Program [2019ZT08Y191]
DNA data storage technology provides long-term stability, high density, and sustainable storage. The development of a Bacillus chassis integrated with an inducible artificially assembled bacterial chromosome enables random data access and demonstrates the stability of data storage through viability assays of spores.
DNA data storage technology may supersede conventional chip or magnetic data storage medium, providing long-term stability, high density, and sustainable storage. Due to its error-correcting capability, DNA data stored in living organisms exhibits high fidelity in information replication. Here we report the development of a Bacillus chassis integrated with an inducible artificially assembled bacterial chromosome to facilitate random data access. We generated three sets of data in the form of DNA sequences using a rudimentary coding system accessible by the regulatory promoter. Sporulated Bacillus harboring the genes were used for long-term storage, where viability assays of spores were subjected to harsh environmental stresses to evaluate the data storage stability. The data accuracy remained above 99% after high temperature and oxidative stress treatment, whereas UV irradiation treatment provided above 96% accuracy. The developed Bacillus chassis and artificial chromosome facilitate the long-term storage of larger datum volume by using other DNA digital encoding and decoding programs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据