期刊
NUTRIENTS
卷 14, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/nu14173593
关键词
coenzyme Q10; spinocerebellar ataxia type 3; locomotor functions; Purkinje cells; muscle atrophy
资金
- Changhua Christian Hospital [104-CCH-ICO-007]
- National Science Council, Taiwan [NSC 102-2314-B-371-002]
- Chung Shan Medical University Hospital [CSH-2015-C-008]
This study demonstrated the protective effect of Coenzyme Q10 (CoQ10) in spinocerebellar ataxia type 3 (SCA3) treatment. Results showed that CoQ10 supplementation significantly improved locomotion in mice and restored the structure of the cerebellar layers. Moreover, CoQ10 facilitated the clearance of mutant ataxin-3 protein through autophagy and restored the expression of antioxidant enzymes. Additionally, CoQ10 treatment prevented muscle weight loss and muscle atrophy in diseased mice.
Coenzyme Q10 (CoQ10), a well-known antioxidant, has been explored as a treatment in several neurodegenerative diseases, but its utility in spinocerebellar ataxia type 3 (SCA3) has not been explored. Herein, the protective effect of CoQ10 was examined using a transgenic mouse model of SCA3 onset. These results demonstrated that a diet supplemented with CoQ10 significantly improved murine locomotion, revealed by rotarod and open-field tests, compared with untreated controls. Additionally, a histological analysis showed the stratification of cerebellar layers indistinguishable from that of wild-type littermates. The increased survival of Purkinje cells was reflected by the reduced abundance of TUNEL-positive nuclei and apoptosis markers of activated p53, as well as lower levels of cleaved caspase 3 and cleaved poly-ADP-ribose polymerase. CoQ10 effects were related to the facilitation of the autophagy-mediated clearance of mutant ataxin-3 protein, as evidenced by the increased expression of heat shock protein 27 and autophagic markers p62, Beclin-1 and LC3II. The expression of antioxidant enzymes heme oxygenase 1 (HO-1), glutathione peroxidase 1 (GPx1) and superoxide dismutase 1 (SOD1) and 2 (SOD2), but not of glutathione peroxidase 2 (GPx2), were restored in 84Q SCA3 mice treated with CoQ10 to levels even higher than those measured in wild-type control mice. Furthermore, CoQ10 treatment also prevented skeletal muscle weight loss and muscle atrophy in diseased mice, revealed by significantly increased muscle fiber area and upregulated muscle protein synthesis pathways. In summary, our results demonstrated biochemical and pharmacological bases for the possible use of CoQ10 in SCA3 therapy.
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