期刊
NUTRIENTS
卷 14, 期 16, 页码 -出版社
MDPI
DOI: 10.3390/nu14163427
关键词
astaxanthin; gastric epithelial cells; Helicobacter pylori; metalloproteinase; mammalian target of rapamycin; phosphatidylinositol 3-kinase; serine; threonine protein kinase B
资金
- National Research Foundation of Korea - Korean Government [NRF-2021R1A2B5B02002353]
Helicobacter pylori infection increases MMP expression and cell invasion, while astaxanthin inhibits MMP expression and cell invasion by suppressing the PI3K/AKT/mTOR signaling pathway.
Helicobacter pylori (H. pylori) increases production of reactive oxygen species (ROS) and activates signaling pathways associated with gastric cell invasion, which are mediated by matrix metalloproteinases (MMPs). We previously demonstrated that H. pylori activated mitogen-activated protein kinase (MAPK) and increased expression of MMP-10 in gastric epithelial cells. MMPs degrade the extracellular matrix, enhancing tumor invasion and cancer progression. The signaling pathway of phosphatidylinositol 3-kinase (PI3K)/serine/threonine protein kinase B (AKT)/mammalian target of rapamycin (mTOR) is associated with MMP expression. ROS activates PIK3/AKT/mTOR signaling in cancer. Astaxanthin, a xanthophyll carotenoid, shows antioxidant activity by reducing ROS levels in gastric epithelial cells infected with H. pylori. This study aimed to determine whether astaxanthin inhibits MMP expression, cell invasion, and migration by reducing the PI3K/AKT/mTOR signaling in H. pylori-infected gastric epithelial AGS cells. H. pylori induced PIK3/AKT/mTOR and NF-kappa B activation, decreased I kappa B alpha, and induced MMP (MMP-7 and -10) expression, the invasive phenotype, and migration in AGS cells. Astaxanthin suppressed these H. pylori-induced alterations in AGS cells. Specific inhibitors of PI3K, AKT, and mTOR reversed the H. pylori-stimulated NF-kappa B activation and decreased I kappa B alpha levels in the cells. In conclusion, astaxanthin suppressed MMP expression, cell invasion, and migration via inhibition of PI3K/AKT/mTOR/NF-kappa B signaling in H. pylori-stimulated gastric epithelial AGS cells.
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