期刊
NUTRIENTS
卷 14, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/nu14214475
关键词
probiotic; intestinal barrier; mucosal immunity; gut microbiota; short-chain fatty acids
资金
- Key R&D Program of Zhejiang Province [2022C02043]
- Hainan Special PhD Scientific Research Foundation of Sanya Yazhou Bay Science and Technology City [HSPHDSRF-2022-04-008]
- 2020 Research Program of Sanya Yazhou Bay Science and Technology City [SKJC-511 2020-02-007]
This study aims to explore the effects of co-cultures from Lactobacillus acidophilus and Bacillus subtilis on the intestinal mucosal barrier. The results showed that FAM supplementation can increase body weight, reduce diarrhea incidence, alleviate mucosal damage, and increase the levels of tight junction proteins, serum diamine oxidase, and antimicrobial peptides. In addition, FAM supplementation also affects the composition of gut microbiota, increases the levels of beneficial bacteria and short-chain fatty acids, and enhances immune cell and SIgA production. FAM has great potential in promoting gut health.
Weaning stress induces intestinal barrier dysfunction and immune dysregulation in mammals. Various interventions based on the modulation of intestinal microbiota have been proposed. Our study aims to explore the effects of co-cultures from Lactobacillus acidophilus and Bacillus subtilis (FAM (R)) on intestinal mucosal barrier from the perspective of metabolic function of gut microbiota. A total of 180 piglets were allocated to three groups, i.e., a control group (C, basal diet), a FAM group (F, basal diet supplemented with 0.1% FAM), and an antibiotic group (A, basal diet supplemented with antibiotic mixtures). Here, we showed FAM supplementation significantly increased body weight and reduced diarrhea incidence, accompanied by attenuated mucosal damage, increased levels of tight junction proteins, serum diamine oxidase (DAO) and antimicrobial peptides. In addition, 16S rRNA sequencing and metabolomic analysis revealed an increase in relative abundance of Clostridiales, Ruminococcaceae, Firmicutes and Muribaculaceae and a significant increase in the total short-chain fatty acids (SCFAs) and butyric acid in FAM-treated piglets. FAM also increased CD4 T cells and SIgA(+) cells in intestinal mucosa and SIgA production in colon contents. Furthermore, FAM upregulated the expression of IL-22, short-chain fatty acid receptors GPR43 and GPR41, aryl hydrocarbon receptor (AhR), and hypoxia-inducible factor 1 alpha (HIF-1 alpha). FAM shows great application prospect in gut health and provides a reference for infant weaning.
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