4.6 Article

ngsLCA-A toolkit for fast and flexible lowest common ancestor inference and taxonomic profiling of metagenomic data

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemical Research Methods

Mutation-Simulator: fine-grained simulation of random mutations in any genome

M. A. Kuehl et al.

Summary: Mutation-Simulator is a tool that allows the introduction of various types of sequence alterations in reference sequences, with fine-grained control over mutation rates for mimicking natural mutation patterns. It can be easily integrated into any development and benchmarking workflow for high-throughput sequencing applications using standard file formats for input and output data.

BIOINFORMATICS (2021)

Article Biochemistry & Molecular Biology

Genome-scale target capture of mitochondrial and nuclear environmental DNA from water samples

Mads Reinholdt Jensen et al.

Summary: The study developed target capture probes for whale shark eDNA sampling, successfully retrieving complete mitochondrial genomes and numerous nuclear loci from aquatic samples. Careful probe design and consideration of target and nontarget sequences are crucial for efficient extraction of population genomic data.

MOLECULAR ECOLOGY RESOURCES (2021)

Article Multidisciplinary Sciences

Late Quaternary dynamics of Arctic biota from ancient environmental genomics

Yucheng Wang et al.

Summary: A large-scale metagenomic analysis of plant and mammal environmental DNA in the circumpolar region over the past 50,000 years shows complex ecological changes, including the extinction of large mammals postglacially and the emergence of modern ecosystems. The study provides insights into the long-term dynamics of Arctic biota at both circumpolar and regional scales, highlighting the power of ancient environmental metagenomics analyses in understanding population histories and long-term ecological dynamics.

NATURE (2021)

Article Ecology

Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures

Karen L. Bell et al.

Summary: In this study, the performance of whole-genome shotgun (WGS) sequencing for species identification and quantitative analysis of pollen was evaluated. WGS showed near 100% accuracy in species identification in mixtures but generated higher rates of false positives compared to rbcL and ITS2 amplicon sequencing. WGS data provided a stronger correlation between pollen grain proportion and sequence read proportion for relative species abundance quantification, but diverged more from a 1:1 relationship.

ECOLOGY AND EVOLUTION (2021)

Article Biochemistry & Molecular Biology

Environmental genomics of Late Pleistocene black bears and giant short-faced bears

Mikkel Winther Pedersen et al.

Summary: Analysis of ancient eDNA has revolutionized our ability to describe biological communities and allows for genomic-scale DNA sequences of closely related species from environmental samples, bringing ancient eDNA into the era of population genomics and phylogenetics.

CURRENT BIOLOGY (2021)

Article Biochemistry & Molecular Biology

Metagenomics: A viable tool for reconstructing herbivore diet

Physilia Y. S. Chua et al.

Summary: Metagenomics can provide comprehensive plant taxonomy information for herbivore diet analysis, with the ability to validate taxonomic assignment through simulated data. However, sample freshness is critical for the performance of metagenomics in diet reconstruction. Both metagenomics and metabarcoding performed similarly in plant taxa identification, with metagenomics also offering additional ecological information through mitochondrial genome analysis.

MOLECULAR ECOLOGY RESOURCES (2021)

Article Biochemical Research Methods

Sensitive protein alignments at tree-of-life scale using DIAMOND

Benjamin Buchfink et al.

Summary: We are at the beginning of a genomic revolution where all known species are planned to be sequenced. The improved version of DIAMOND allows for quick tree-of-life scale protein alignments.

NATURE METHODS (2021)

Article Multidisciplinary Sciences

Unearthing Neanderthal population history using nuclear and mitochondrial DNA from cave sediments

Benjamin Vernot et al.

Summary: This study demonstrates the importance of using nuclear DNA extracted from sediments for studying the population history of ancient hominins. Population replacement in northern Spain and radiation events in Neanderthal history during the Late Pleistocene were identified through analysis of cave deposits in western Europe and southern Siberia.

SCIENCE (2021)

Article Biology

Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3

Francesco Beghini et al.

Summary: Culture-independent analyses of microbial communities have advanced significantly in the past decade, particularly due to the emergence of Shotgun Metagenomics. bioBakery 3, a newly developed toolset, enhances the accuracy and scope of taxonomic, functional, and phylogenetic profiling in microbial community studies by providing an expanding set of reference sequences.
Article Biochemical Research Methods

PuffAligner: a fast, efficient and accurate aligner based on the Pufferfish index

Fatemeh Almodaresi et al.

Summary: PuffAligner is a fast, accurate, and versatile aligner that can produce highly sensitive alignments on large collections of reference sequences, striking a desirable balance between time, space, and accuracy tradeoffs.

BIOINFORMATICS (2021)

Article Biotechnology & Applied Microbiology

Terminating contamination: large-scale search identifies more than 2,000,000 contaminated entries in GenBank

Martin Steinegger et al.

GENOME BIOLOGY (2020)

Review Biotechnology & Applied Microbiology

A review on the applications and recent advances in environmental DNA (eDNA) metagenomics

Deviram Garlapati et al.

REVIEWS IN ENVIRONMENTAL SCIENCE AND BIO-TECHNOLOGY (2019)

Article Biotechnology & Applied Microbiology

Improved metagenomic analysis with Kraken 2

Derrick E. Wood et al.

GENOME BIOLOGY (2019)

Article Biotechnology & Applied Microbiology

KrakenUniq: confident and fast metagenomics classification using unique k-mer counts

F. P. Breitwieser et al.

GENOME BIOLOGY (2018)

Article Biochemical Research Methods

gargammel: a sequence simulator for ancient DNA

Gabriel Renaud et al.

BIOINFORMATICS (2017)

Review Biochemistry & Molecular Biology

Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

Kristy Deiner et al.

MOLECULAR ECOLOGY (2017)

Article Biochemical Research Methods

Complex heatmaps reveal patterns and correlations in multidimensional genomic data

Zuguang Gu et al.

BIOINFORMATICS (2016)

Article Multidisciplinary Sciences

Postglacial viability and colonization in North America's ice-free corridor

Mikkel W. Pedersen et al.

NATURE (2016)

Article Multidisciplinary Sciences

Fast and sensitive taxonomic classification for metagenomics with Kaiju

Peter Menzel et al.

NATURE COMMUNICATIONS (2016)

Article Biochemical Research Methods

MEGAN Community Edition - Interactive Exploration and Analysis of Large-Scale Microbiome Sequencing Data

Daniel H. Huson et al.

PLOS COMPUTATIONAL BIOLOGY (2016)

Article Biodiversity Conservation

Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity

Philip Francis Thomsen et al.

BIOLOGICAL CONSERVATION (2015)

Article Multidisciplinary Sciences

Genome sequence of a 45,000-year-old modern human from western Siberia

Qiaomei Fu et al.

NATURE (2014)

Review Veterinary Sciences

Wildlife health investigations: needs, challenges and recommendations

Marie-Pierre Ryser-Degiorgis

BMC VETERINARY RESEARCH (2013)

Article Biochemical Research Methods

Fast gapped-read alignment with Bowtie 2

Ben Langmead et al.

NATURE METHODS (2012)

Article Biochemical Research Methods

The Sequence Alignment/Map format and SAMtools

Heng Li et al.

BIOINFORMATICS (2009)

Article Biochemical Research Methods

Fast and accurate short read alignment with Burrows-Wheeler transform

Heng Li et al.

BIOINFORMATICS (2009)