Single cell atlas of spinal cord injury in mice reveals a pro-regenerative signature in spinocerebellar neurons

After spinal cord injury, tissue distal to the lesion contains undamaged cells that could support or augment recovery. Targeting these cells requires a clearer understanding of their injury responses and capacity for repair. Here, we use single nucleus RNA sequencing to profile how each cell type in the lumbar spinal cord changes after a thoracic injury in mice. We present an atlas of these dynamic responses across dozens of cell types in the acute, subacute, and chronically injured spinal cord. Using this resource, we find rare spinal neurons that express a signature of regeneration in response to injury, including a major population that represent spinocerebellar projection neurons. We characterize these cells anatomically and observed axonal sparing, outgrowth, and remodeling in the spinal cord and cerebellum. Together, this work provides a key resource for studying cellular responses to injury and uncovers the spontaneous plasticity of spinocerebellar neurons, uncovering a potential candidate for targeted therapy.

Automated summary

After spinal cord injury, certain cells in the injured area show potential for regeneration. By analyzing different cell types in the spinal cord of mice, this study identifies spinal neurons that express regeneration characteristics and describes their anatomical features. The study provides a valuable resource for understanding cellular responses to injury and highlights the spontaneous plasticity of spinocerebellar neurons as a potential target for targeted therapy.