期刊
VIRUSES-BASEL
卷 14, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/v14091962
关键词
tobacco mosaic virus; RNA virus; RNA replication; translation
类别
资金
- Japan Society for the Promotion of Science [20K15495, 22K14896]
This study demonstrates that small RNA fragments derived from the NR region can inhibit the replication of TMV and affect the translation of mRNA.
Replication proteins of tobacco mosaic virus (TMV), a positive-sense RNA virus, cotranslationally bind to a 5'-proximal similar to 70-nucleotide (nt) region of the genomic RNA, referred to as the nuclease-resistant (NR) region for replication template selection. Therefore, disruption of the interaction between the viral replication proteins and viral genomic RNA is expected to inhibit the replication of TMV. In this study, we demonstrate that the addition of small RNA fragments (18-33 nts in length) derived from different regions within the NR region inhibit the binding of TMV replication proteins to viral RNA and TMV RNA replication in a cell-free system. Intriguingly, some of the small RNA fragments also inhibited the translation of mRNA in a sequence-nonspecific manner. These results highlight the pleiotropic roles of the 5'-proximal region of the TMV genome.
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