期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 38, 期 12, 页码 -出版社
SPRINGER
DOI: 10.1007/s11274-022-03425-y
关键词
Bee venom-loaded chitosan nanoparticles; Cryptococcus neoformans; Kodamaea ohmeri; Candida albicans; Biofilm formation; Yeast-hyphae transition
资金
- Science, Technology & Innovation Funding Authority (STDF)
- Egyptian Knowledge Bank (EKB)
This study aimed to develop a novel agent to control fungal pathogens by screening honeybee products against unicellular fungal (UCF) pathogens. Bee venom was found to exhibit inhibitory effect against the UCF pathogens. The protein contents of crude bee venom were separated and visualized, and Apis mellifera loaded chitosan nanoparticles were prepared. The nanoparticles showed improved inhibitory effect against the UCF pathogens, suppressing biofilm formation and yeast to hyphal transition. This suggests that bee venom loaded chitosan nanoparticles are a promising natural compound for fungal pathogens treatment.
The prevalence of opportunistic human fungal pathogens is increasing worldwide, and antimicrobial resistance is one of the greatest medical challenges the world faces. Therefore, this study aimed to develop a novel agent to control fungal pathogens. The honeybee products (honey, royal jelly, propolis, bee bread, and bee venom) were screened against unicellular fungal (UCF) pathogens (Cryptococcus neoformans, Kodamaea ohmeri, and Candida albicans) and the bee venom was only exhibited an inhibitory effect against them. The protein contents of crude bee venom were separated using the gel filtration technique into eight fractions which were visualized on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to confirm the presence of five bands with molecular weights of 65, 43, 21, 15, and 3 KDa. Bee venom (BV) of Apis mellifera loaded chitosan nanoparticles were prepared by the ionotropic gelation method. The encapsulation efficiency%, average size, zeta potentials, and surface appearance by Transmission electron microscope (TEM) were evaluated for the prepared nanoparticles. The minimum inhibitory concentration (MIC) of crude BV and BV loaded chitosan nanoparticles (BV-CNPs) was evaluated against the offer mentioned UCF where the MIC values of crude BV were 6.25, 3.12 & 6.25 while MIC values in the case of BV-CNPs were decreased to 3.12, 3.12 & 1.56 mg/ml against C. neoformans, K. ohmeri and C. albicans, respectively. Also, the results showed that BV-CNPs suppressed the biofilm formation as well as yeast to hyphal transition formed by the examined UCF. These results revealed that BV-CNPs are a promising natural compound for fungal pathogens treatment.
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