4.8 Article

Microbial life strategy with high rRNA operon copy number facilitates the energy and nutrient flux in anaerobic digestion

期刊

WATER RESEARCH
卷 226, 期 -, 页码 -

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2022.119307

关键词

Anaerobic digestion; Energy and nutrient flux; Microbial life strategy; rRNA operon copy number; rRNA operon expression; Straw

资金

  1. Open Found of Key Laboratory of Environmental and Applied Microbiology CAS [11050004GB]
  2. China Biodiversity Observation Networks (Sino BON)

向作者/读者索取更多资源

This study investigated the relationship between microbial life strategy and energy and nutrient flux in anaerobic digestion (AD). The results showed that there was a positive correlation between microbial rrn copy number and CH4 daily production, and this correlation was weakened when feedstock complexity was reduced. Furthermore, the mean rrn expression ratio of microbial communities also exhibited similar patterns. The findings reveal a novel relationship between microbial community life strategy and the energy and nutrient flux during AD.
Microbial life strategy, reflected by rRNA operon (rrn) copy number, determines microbial ecological roles. However, the relationship between microbial life strategy and the energy and nutrient flux in anaerobic digestion (AD) remains elusive. This study investigated microbial rrn copy number and expression ratio using amplicon sequencing of 16S rRNA gene and 16S rRNA, and monitored CH4 daily production to approximate the status of energy and nutrient flux in semi-continuous AD. A significantly positive correlation between the mean rrn copy number of microbial communities in digestate and CH4 daily production was detected in the control treatment fed swine manure. The reduced feedstock complexity, by replacing parts of swine manure with fructose or apple waste, weakened the correlation. When feedstock complexity was increased again, the correlation was strengthened again. Similar results were detected in mean rrn expression ratio of microbial communities. The responses of mean rrn copy number and expression ratio of communities to feedstock addition differed between the reduced feedstock complexity and the control treatment, as well as between in digestate and in straw. Our findings reveal a novel relationship between microbial community life strategy and the energy and nutrient flux, and the roles of feedstock characteristics therein in AD.

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