4.8 Article

Metagenomic insights into the symbiotic relationship in anammox consortia at reduced temperature

期刊

WATER RESEARCH
卷 225, 期 -, 页码 -

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2022.119184

关键词

Reduced temperature; Anammox bacteria; Heterotrophic bacteria; Co-occurrence network; Metabolism interaction

资金

  1. National Natural Science Foundation of China [41977142]
  2. Open Research Fund Program of State Environmental Protection Key Laboratory of Food Chain Pollution Control [FC2022YB08]

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This study found that reducing the temperature promoted the production of larger granular sludge in a laboratory-scale anammox reactor. The researchers also discovered potential interactions between AnAOB and heterotrophic bacteria through network analysis and metagenomics. These interactions help maintain the stability of the anammox reactor.
Anammox as a promising biological nitrogen removal technology has attracted much attention. However, cold temperature would limit its wide application and little is known about the microbial interactions between anammox bacteria (AnAOB) and heterotrophic bacteria at cold temperature. Here, we observed reduced tem-perature (25-15 degrees C) promoted the secretion of EPS and thus stimulated bigger size of granular sludge in a laboratory-scale anammox reactor. We further combined co-occurrence network analysis and genome-centered metagenomics to explore the potential interactions between AnAOB and heterotrophic bacteria. Network anal-ysis suggested 22 out of 25 positively related species were reported as definite heterotrophic bacteria in sub-network of AnAOB. Genome-centered metagenomics analysis yielded 23 metagenomic assembly genomes (MAGs), and we found that Acidobacteriota-affiliated bacteria could biosynthesize most polysaccharides (PS) precursors and contain the most glycosyltransferases and transporters to facilitate exopolysaccharides biosyn-thesis, together with partial PS precursors produced by AnAOB. AMX1 as the only anammox genome could synthesize most amino acids and cross feed with some heterotrophs to affect the extracellular protein function. Additionally, Bacteroidota, Planctomycetota, Chloroflexota, and Proteobacteria could contribute folate and molybdopterin cofactor for AMX1 to benefit their activity and growth. Superphylum Patescibacteria could survive by cross-feeding with AnAOB and heterotrophic organisms about organic compounds (Glyceraldehyde-3P and lactate). These cross-feedings maintained the stability of anammox reactor performance and emphasize the importance of heterotrophs in anammox system at reduced temperature.

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