期刊
JOURNAL OF MASS SPECTROMETRY
卷 51, 期 11, 页码 1030-1035出版社
WILEY-BLACKWELL
DOI: 10.1002/jms.3827
关键词
airbrush; automatic sprayer; biofilms; MALDI MSI; matrix application
资金
- Engineer Research and Development Center-Construction Engineering Research Laboratory (ERDC-CERL) [W9132T-15-2-0006]
- National Institute of Allergy and Infectious Diseases [R01 AI113219]
- National Science Foundation Graduate Research Fellowship Program
- Springborn Fellowship
- Training Program at ChemistryInterface with Biology [T32 GM070421]
Matrix-assisted laser desorption/ionization imaging of biofilms cultured on agar plates is challenging because of problems related to matrix deposition onto agar. We describe a one-step, spray-based application of a 2,5-dihydroxybenzoic acid solution for direct matrix-assisted laser desorption/ionization imaging of hydrated Bacillus subtilis biofilms on agar. Using both an optimized airbrush and a home-built automatic sprayer, region-specific distributions of signaling metabolites and cannibalistic factors were visualized from B. subtilis cells cultivated on biofilm-promoting medium. The approach provides a homogeneous, relatively dry coating on hydrated samples, improving spot to spot signal repeatability compared with sieved matrix application, and is easily adapted for imaging a range of agar-based biofilms. Copyright (C) 2016 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据