4.6 Review

Omics tools enabling vaccine discovery against fasciolosis

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Parasitology

A global phosphoproteomics analysis of adult Fasciola gigantica by LC-MS/MS

Ming Pan et al.

Summary: This study has performed the first global phosphoproteomics analysis of adult Fasciola gigantica, identifying 635 phosphorylated proteins involved in various biological processes. These proteins are predominantly located in cellular components and have functions related to binding and catalytic activity.

PARASITOLOGY RESEARCH (2022)

Article Microbiology

Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins

Carolina De Marco Verissimo et al.

Summary: This study discovered the mechanism by which helminth parasite Fasciola hepatica resists complement attack, including preventing lectin binding and secretion of protease inhibitors, revealing new interventions against parasitic infections.

PLOS PATHOGENS (2022)

Article Immunology

Developmental Regulation and Functional Prediction of microRNAs in an Expanded Fasciola hepatica miRNome

Caoimhe M. Herron et al.

Summary: This study provides the first data on developmental miRNA regulation and host gene regulation in liver flukes, expanding our understanding of the F. hepatica miRNome.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

Article Immunology

Targeting Secreted Protease/Anti-Protease Balance as a Vaccine Strategy against the Helminth Fasciola hepatica

Krystyna Cwiklinski et al.

Summary: This study reports a vaccine strategy targeting the parasite's protease/anti-protease balance. A vaccine cocktail containing three recombinant stefins and a Kunitz-type inhibitor was evaluated in two sheep trials. Although fluke burden was not reduced, the vaccinated animals showed significantly greater weight gain and a significant reduction in egg viability. Multivariate regression analysis indicated that vaccination and increased levels of antibodies specific for the parasite's peptidase inhibitors were positive indicators for increased weight gain and normal levels of hemoglobin.

VACCINES (2022)

Article Biochemistry & Molecular Biology

Fasciola hepatica Gastrodermal Cells Selectively Release Extracellular Vesicles via a Novel Atypical Secretory Mechanism

Adam P. S. Bennett et al.

Summary: This study used laser microdissection to analyze the proteome of the gastrodermal cells in the liver fluke. The results showed that these cells are the main source of secreted proteins and may release extracellular vesicles. The study also highlighted the importance of the gastrodermal cells in nutrient acquisition and immunomodulation.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Immunology

Applications of platform technologies in veterinary vaccinology and the benefits for one health

Gary Entrican et al.

Summary: The animal-human interface has been crucial in the advancements of vaccinology for the past two centuries. Traditional veterinary vaccines have been successful, but alternative approaches are necessary now. The new generation of vaccine platform technologies can elicit high-titre antibody responses and induce CD4 + T cell and/or CD8 + T cell responses.

VACCINE (2022)

Article Immunology

Proteomic Analysis of Extracellular Vesicles From Fasciola hepatica Hatching Eggs and Juveniles in Culture

Maria Trelis et al.

Summary: The protein cargo of extracellular vesicles (EVs) from different developmental stages of Fasciola hepatica, including embryonated eggs and newly-excysted juvenile (NEJ) flukes, have been analyzed. The analysis revealed qualitative differences in composition, reflecting the intense activity of NEJs at this early stage, with several proteins involved in membrane traffic and cell physiology.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

Article Biotechnology & Applied Microbiology

Stage-specific miRNAs regulate gene expression associated with growth, development and parasite-host interaction during the intra-mammalian migration of the zoonotic helminth parasite Fasciola hepatica

Alison Ricafrente et al.

Summary: This study describes the miRNAs expressed by different developmental stages of the parasitic worm Fasciola hepatica and identifies new genes and miRNAs involved in parasite development and metabolic pathways. These findings provide novel insight into the regulation of helminth parasite development and have implications for therapeutic development.

BMC GENOMICS (2022)

Article Multidisciplinary Sciences

Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature

Jana Ilgova et al.

Summary: Fasciola hepatica is a global parasite of livestock that also causes a neglected zoonosis in humans. This study demonstrates significant molecular changes in F. hepatica eggs when cultured at the physiological temperature of the definitive host. The transcriptome and proteome of the eggs underwent noticeable changes, and the peptidase profile was considerably modified on both levels.

SCIENTIFIC REPORTS (2022)

Review Cell Biology

Regulation of Tubulin Gene Expression: From Isotype Identity to Functional Specialization

Ivana Gasic

Summary: The genomes of higher eukaryotes contain a large tubulin gene superfamily with various isotypes. The expression and regulation of these isotypes in different cell types, as well as their impact on the biomechanical properties of the microtubule cytoskeleton, are still poorly understood. Recent studies shed light on the complex regulatory pathways involved in the expression and function of tubulin isotypes, particularly during development and in specialized cells. This review summarizes our current understanding of the regulation of tubulin isotype expression and its importance in building a customized microtubule network for cellular needs.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2022)

Article Veterinary Sciences

Bovine Natural Antibody Relationships to Specific Antibodies and Fasciola hepatica Burdens after Experimental Infection and Vaccination with Glutathione S-Transferase

Gemma Zerna et al.

Summary: Currently, there is no vaccine to control Fasciola hepatica infections in livestock. However, recent research suggests that natural antibodies may play a role in amplifying specific antibodies and killing the parasites. A study on cattle showed that parasite infection and vaccination have different effects on the production of natural antibodies, and certain natural antibodies were correlated with increased parasite burdens. Furthermore, the liver fluke may evade the host's immune system by utilizing surface-bound natural antibodies.

VETERINARY SCIENCES (2022)

Review Parasitology

Insights into Fasciola hepatica Juveniles: Crossing the Fasciolosis Rubicon

Javier Gonzalez-Miguel et al.

Summary: Understanding the molecular interactions between parasites and host tissues is crucial for developing effective parasite control strategies. Research on the early events of fasciolosis caused by Fasciola hepatica is limited, with most studies focusing on adult worms.

TRENDS IN PARASITOLOGY (2021)

Article Biotechnology & Applied Microbiology

Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver

Krystyna Cwiklinski et al.

Summary: The migration of immature Fasciola hepatica parasites within the liver is associated with an increase in protein production, expression of signalling pathways, and neoblast proliferation that drive their rapid growth and development. The secretion of a defined set of molecules, particularly cathepsin L peptidases, peptidase inhibitors, saponins, immune regulators, and antioxidants, allow the parasite to negotiate the liver micro-environment, immune attack, and increasing levels of oxidative stress.

BMC GENOMICS (2021)

Article Parasitology

Differential expression of microRNAs and tRNA fragments mediate the adaptation of the liver fluke Fasciola gigantica to its intermediate snail and definitive mammalian hosts

Rui-Si Hu et al.

Summary: The study investigated the transcriptional changes of small RNAs during different developmental stages of the tropical liver fluke Fasciola gigantica, revealing important roles of miRNAs and tRFs in parasite development. Furthermore, comparison of miRNA composition between F. gigantica and Fasciola hepatica showed a high level of conservation with some distinct differences, which could be used for species differentiation.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2021)

Review Immunology

Pathogenicity and virulence of the liver flukes Fasciola hepatica and Fasciola Gigantica that cause the zoonosis Fasciolosis

Richard Lalor et al.

Summary: Fasciolosis caused by liver flukes is an important neglected parasitic disease with high transmissibility and adaptability. The parasite's ability to survive and reproduce within their mammalian host is mainly influenced by their high frequency of non-synonymous polymorphisms in genes and excretory-secretory molecules. Through various mechanisms, liver flukes can persist for years within their host and spread rapidly.

VIRULENCE (2021)

Article Infectious Diseases

High-quality reference genome of Fasciola gigantica: Insights into the genomic signatures of transposon-mediated evolution and specific parasitic adaption in tropical regions

Xier Luo et al.

Summary: Fascioliasis is a neglected zoonotic tropical disease that imposes a significant economic burden. The Pacbio assembly of the reference genome for F. gigantica reported in the study significantly improved assembly and gene annotation quality, and revealed evidence of transposon-mediated evolution.

PLOS NEGLECTED TROPICAL DISEASES (2021)

Review Immunology

Autonomous Non Antioxidant Roles for Fasciola hepatica Secreted Thioredoxin-1 and Peroxiredoxin-1

Amber Dorey et al.

Summary: Research has shown that the Prx1 and Trx1 of Fasciola hepatica play important roles in stress responses, not only acting as antioxidants, but also modulating host immune responses and inflammation.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

Article Veterinary Sciences

Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep

Rafael Zafra et al.

Summary: In this study, sheep were vaccinated with two different vaccines containing a cocktail of antigens and formulated with different adjuvants. The results showed that the first vaccine significantly reduced fluke burden, decreased egg output, and alleviated hepatic lesions. Additionally, animals in the first vaccine group exhibited higher levels of IgG1 and IgG2 in the serological study, supporting the hypothesis of protection found in this group.

VETERINARY RESEARCH (2021)

Review Immunology

Viral targets for vaccines against COVID-19

Lianpan Dai et al.

Summary: The urgent need for vaccines to control the COVID-19 pandemic has led to the rapid development of multiple vaccine candidates, with some showing positive results in late-stage clinical trials. This article discusses the viral elements used in these candidates, the reasons why they are good targets for the immune system, and their implications for protective immunity. Dai and Gao highlight the importance of selecting the right viral targets in vaccine development to ensure an effective immune response and the overall safety and efficacy of the vaccine.

NATURE REVIEWS IMMUNOLOGY (2021)

Article Biochemistry & Molecular Biology

Adaptive Radiation of the Flukes of the Family Fasciolidae Inferred from Genome-Wide Comparisons of Key Species

Young-Jun Choi et al.

MOLECULAR BIOLOGY AND EVOLUTION (2020)

Review Parasitology

Drug resistance in liver flukes

I Fairweather et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE (2020)

Article Chemistry, Multidisciplinary

Draft Genome of the Liver Fluke Fasciola gigantica

Tripti Pandey et al.

ACS OMEGA (2020)

Review Parasitology

Extracellular vesicles: new targets for vaccines against helminth parasites

Claire Drurey et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2020)

Article Parasitology

The cellular and molecular origins of extracellular vesicles released by the helminth pathogen, Fasciola hepatica

Adam P. S. Bennett et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2020)

Review Parasitology

The potential for vaccines against scour worms of small ruminants

Collette Britton et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2020)

Article Infectious Diseases

Fasciola hepatica Serine Protease Inhibitor Family (Serpins): Purposely crafted for regulating host proteases

Carolina De Marco Verissimo et al.

PLOS NEGLECTED TROPICAL DISEASES (2020)

Review Immunology

Fasciola hepatica-Derived Molecules as Regulators of the Host Immune Response

Sinead Ryan et al.

FRONTIERS IN IMMUNOLOGY (2020)

Article Parasitology

The rational simplification of a recombinant cocktail vaccine to control the parasitic nematode Teladorsagia circumcincta

Alasdair J. Nisbet et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2019)

Article Parasitology

Global serum proteomic changes in water buffaloes infected with Fasciola gigantica

Fu-Kai Zhang et al.

PARASITES & VECTORS (2019)

Article Genetics & Heredity

Comparative genomics of the major parasitic worms

Avril Coghlan et al.

NATURE GENETICS (2019)

Article Parasitology

Advances in Fasciola hepatica research using 'omics' technologies

Krystyna Cwiklinski et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2018)

Article Parasitology

Profiling G protein-coupled receptors of Fasciola hepatica identifies orphan rhodopsins unique to phylum Platyhelminthes

Paul McVeigh et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE (2018)

Article Multidisciplinary Sciences

Visualizing the GPCR Network: Classification and Evolution

Geng-Ming Hu et al.

SCIENTIFIC REPORTS (2017)

Article Biochemistry & Molecular Biology

Unexpected Activity of a Novel Kunitz-type Inhibitor INHIBITION OF CYSTEINE PROTEASES BUT NOT SERINE PROTEASES

David Smith et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2016)

Article Biochemical Research Methods

Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host

Alessandra Ravida et al.

MOLECULAR & CELLULAR PROTEOMICS (2016)

Article Multidisciplinary Sciences

Across intra-mammalian stages of the liver fluke Fasciola hepatica: a proteomic study

Lucia Sanchez Di Maggio et al.

SCIENTIFIC REPORTS (2016)

Article Parasitology

Fasciola hepatica vaccine: We may not be there yet but we're on the right road

Veronica Molina-Hernandez et al.

VETERINARY PARASITOLOGY (2015)

Review Parasitology

Liver fluke vaccines in ruminants: strategies, progress and future opportunities

Hayley Toet et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2014)

Article Immunology

Type 2 immunity and wound healing: evolutionary refinement of adaptive immunity by helminths

William C. Gause et al.

NATURE REVIEWS IMMUNOLOGY (2013)

Article Parasitology

Exploring the Fasciola hepatica tegument proteome

R. Alan Wilson et al.

INTERNATIONAL JOURNAL FOR PARASITOLOGY (2011)

Letter Parasitology

Worm secretory molecules are causing alarm

Mark W. Robinson et al.

TRENDS IN PARASITOLOGY (2010)

Review Pharmacology & Pharmacy

Microtubules as antiparasitic drug targets

B. J. Fennell et al.

EXPERT OPINION ON DRUG DISCOVERY (2008)

Article Parasitology

Parasite vaccines - a reality?

JP Dalton et al.

VETERINARY PARASITOLOGY (2001)