期刊
TALANTA
卷 247, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2022.123543
关键词
SARS-CoV-2 spike S1 protein; COVID-19; Electrochemiluminescence; Immunosensor; Wastewater
资金
- Spanish Ministerio de Ciencia e Innovacion [PID2020-116728RB-I00]
- Comunidad Autonoma de Madrid [SI3/PJI/2021-00341, 2017-T1/BIO-5435, 2021-5A/BIO-20943, S2018/NMT-4349]
Given the importance of fast, efficient, and cost-effective detection of SARS-CoV-2 in wastewater to control the pandemic, a new method based on a carbon nanodots-amplified electrochemiluminescence immunosensor has been proposed. The immunosensor showed high specificity and stability, with a detection limit of 1.2 pg/mL, and successfully detected SARS-CoV-2 in river and urban wastewater. This sensing platform provides a feasible and reliable tool for early virus detection and protection of the population.
Given the great utility that having fast, efficient and cost-effective methods for the detection of SARS-CoV-2 in wastewater can have in controlling the pandemic caused by this virus, the development of new dependable and specific SARS-CoV-2 coronavirus sensing devices to be applied to wastewater is essential to promote public health interventions. Therefore, herein we propose a new method to detect SARS-CoV-2 in wastewater based on a carbon nanodots-amplified electrochemiluminescence immunosensor for the determination of the SARS-CoV-2 Spike S1 protein. For the construction of the immunosensor, N-rich carbon nanodots have been synthetized with a double function: to contribute as amplifiers of the electrochemiluminescent signal in presence of [Ru (bpy)3]2+ and as antibody supports by providing functional groups capable of covalently interacting with the SARS-CoV-2 Spike S1 antibody. The proposed ECL immunosensor has demonstrated a high specificity in presence of other virus-related proteins and responded linearly to SARS-CoV-2 Spike S1 concentration over a wide range with a limit of detection of 1.2 pg/mL. The immunosensor has an excellent stability and achieved the detection of SARS-CoV-2 Spike S1 in river and urban wastewater, which supplies a feasible and reliable sensing platform for early virus detection and therefore to protect the population. The detection of SARS-CoV-2 Spike S1 in urban wastewater can be used as a tool to measure the circulation of the virus in the population and to detect a possible resurgence of COVID-19.
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