期刊
TALANTA
卷 253, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2022.123990
关键词
Palindromic DNA nanospheres; Gold nanoparticles; Telomerase; SYBR Green I; Naked-eye platform; Colorimetric assay
A label-free platform utilizing target-triggered hybridization and inhibition of gold nanoparticle aggregation was developed for naked-eye detection of telomerase activity. This method offers a cost-effective approach for cancer diagnosis and identification of breast cancer phenotypes.
A versatile naked-eye colorimetric assay platform is a desirable format for rapid analysis of disease-specific biomarkers, but is constrained by a complicated labeling process and false-positive signal readout. Target -triggered label-free palindromic DNA nanospheres (P-DNANS) were developed for the first time for the visual detection of telomerase activity. Telomerase recognition and extension trigger a hybridization event that yields Y-shaped double helices as core motifs. Y-shaped DNAs were linked by their palindromic sticky ends and P-DNANS were subsequently assembled with SYBR Green I (SG) in the helix. The SG-induced aggregation of gold nanoparticles is inhibited, and thus the red color can be observed by the naked eye. This label-free platform offers a cost-effective method for the cancer diagnosis and identification of breast cancer phenotypes.
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