A novel ESIPT-based fluorescent probe with dual recognition sites for the detection of hydrazine in the environmental water samples and in-vivo bioimaging

Hydrazine (N2H4), an important chemical intermediate, has been widely used in industrial production and agricultural life, but it has also caused environmental pollution. A novel ESIPT-based fluorescent probe with dual recognition sites, 2-(benzothiazole-2-yl)-1,4-imphenyl bis 4-bromobutyric acid (BRBA), was developed to selectively detect N2H4 under complex conditions. BRBA exhibits accurate detection for N2H4 with a good linear relationship ranging from 0 to 150 mu M, and the LOD can reach 0.1 mu M. Importantly, taking advantage of low cytotoxicity and a large Stokes shift, BRBA can be utilized to monitor environmental water samples and successfully applied to imaging HeLa cells and zebrafish.

Automated summary

A novel fluorescent probe, BRBA, has been developed for selective detection of hydrazine under complex conditions. The probe exhibits low cytotoxicity and a large Stokes shift, making it suitable for monitoring environmental water samples and imaging cells.