期刊
SMALL
卷 18, 期 45, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202204859
关键词
affinity; biological silence; lateral flow immunoassays; nano-tracers; surface-enhanced Raman scattering
类别
资金
- National Key Research and Development Program of China [2018YFE0127000]
- National Science Foundation of China [21675127, 31972150]
- Key Industries Innovation Chain Project of Shaanxi Province [2019ZDLSF07-08]
- Natural Science Foundation Project in Guangdong Provincial [2020A1515010778]
- Qinghai Provincial Key Laboratory of Qinghai-Tibet Plateau Biological Resource [2022-ZJ-Y18]
This study demonstrates the potential of stimulated surface-enhanced Raman scattering (SERS) combined with engineered nano-tracer in lateral flow immunoassays (LFIAs). The engineered core-shell multifunctional nano-tracer shows enhanced coupling efficiency, highly retained affinity, and reinforced colloid stability, leading to improved analysis performance.
Stimulated surface-enhanced Raman scattering (SERS) in combination with engineered nano-tracer offers extraordinary potential in lateral flow immunoassays (LFIAs). Nonetheless, the investigation execution of SERS-LFIA is often compromised by the intricacy and overlap of the Raman fingerprint spectrum as well as the affinity-interference of nano-tracer to antibody. To circumvent these critical issues, an engineered core-shell multifunctional nano-tracer (named APNPs) with precise control of the size of nano-core (AuNPs) and coating of the nano-shell (Prussian blue nanomaterials) is prepared for SERS-LFIA via a modified enlarging particle size and coating modification strategy. Importantly, this nano-tracer exhibits enhanced coupling efficiency, highly retained affinity, reinforced colloid stability, and unique SERS signal (2156 cm(-1)) in the silent region (1800-2800 cm(-1)) with high signal-to-background ratio simultaneously, all of which are beneficial to the enhancement of the analysis performance. With a proof-of-concept demonstration for detection of ractopamine (RAC), a dual-pattern LFIA that synergizes both the enlarged particle size and coating modification supported colorimetric/biological silence Raman dual-response (coined as the ECCRD assay) is demonstrated by integrating APNPs with the competitive-type immunoreaction. This research may contribute to the rational design of multifunctional nano-tracer, and the ECCRD assay can be expanded for a wide spectrum of applications in environmental monitoring and biomedical diagnosis.
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