期刊
SMALL
卷 18, 期 49, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202204485
关键词
2-photon polymerization; 3D cell culture; brain cancer; epidermal growth factor receptor; microtubes
类别
资金
- Brain Tumour Charity [ET_2019_2_10470]
- Erasmus MC Academic Centre of Excellence Tumor Immunology and Immune Therapy
- TU Delft Bioengineering Institute MSc Grant
A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. This study developed a 3D-engineered scaffold as a cell culture model system to study patient-derived glioma cells. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells and intracellular trafficking.
A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies.
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