期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 366, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.132021
关键词
DNA walker; A549 cells; Electrochemistry; Competitive sensor; Signal amplification
资金
- National Natural Science Foundation of China [21974019, 92061121, 82061148012, 82027806, 22074130]
- National High-tech R&D Program and National Key Research & Development Program of China [2017YFA0205301]
- Primary Research & Development Plan of Jiangsu Province [BE2019716]
- Zhongyuan Thousand Talents Plan-Science and Technology Innovation Leading Talents Project [204200510030]
- Henan Provincial Science and Technology Innovation Team [C20150026]
- Nanhu Scholars Program of XYNU
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20-0142]
This study reports a DNA walker driven by endonuclease Nt.BbvC I for highly sensitive detection of tumor cells. By modifying the electrode surface and using magnetic beads, an ultra-high sensitivity electrochemical cell analysis is achieved.
The fragments of molecular motors can be transported and operated autonomously along a track. Most molecular walker systems are assembled from DNA building modules, exhibiting great potential applications in biocomputing, drug transport, sensing, etc. Hereby, we report a DNA walker driven by endonuclease Nt.BbvC I that can autonomously induce signal off sensing platform for highly sensitive detection of tumor cells. DNA density and trajectory conformation can affect DNA walker properties. Molybdenum selenide@gold nanoparticles (MoSe2@AuNPs) are modified on the electrode surface as a scaffold to accommodate more hairpin probe (H1) and provide a good interface for DNA walking. Magnetic beads are used to enrich target tumor cells, release single-stranded DNA (S1) and trigger DNA walker-based amplification strategy. Upon addition of tumor cells, more DNA-methylene blue (DNA-MB) is cracked from the electrode surface. Benefiting from efficient electrode modification, magnetic enrichment, DNA walker and enzyme-assisted amplification, an ultra-high sensitivity electrochemical cell analysis is realized, with a detection limit of 2 cells mL-1. More importantly, this DNA walker strategy is available for tumor cell analysis in clinical samples, suggesting the potential diagnostic applications.
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