期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 371, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.132591
关键词
ECL-RET; ZnONFs@PDA-sCuO; Procalcitonin detection
资金
- National Natural Science Founda-tion of China
- National Key Scientific Instrument and Equip-ment Development Project of China
- [21607055]
- [21675063]
- [21775054]
- [21505051]
- [21575050]
- [21777056]
- [21627809]
This work presents a dual-quenching electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor for sensitive analysis of procalcitonin (PCT). The sensor utilizes polydopamine coated ZnO nanoflowers loaded with small-sized copper oxide nanoparticles to achieve double quenching effects, enhancing the emission performance of g-C3N4. The biosensor showed superior linear detection range and sensitive detection limit, providing a new technical means for the diagnosis of serious diseases.
This work presents a dual-quenching electrochemiluminescence resonance energy transfer (ECL-RET) immu-nosensor on account of the double quenching effects of polydopamine coated ZnO nanoflowers (ZnONFs@PDA) loaded with small-sized copper oxide nanoparticles (sCuO), named ZnONFs@PDA-sCuO, towards gold den-drite@polypyrrole core-shell nanoparticles (AuD@PPy) enhanced g-C3N4 (g-C3N4-AuD@PPy) for sensitive analysis of procalcitonin (PCT). To be specific, AuD@PPy with core-shell structure were loaded onto g-C3N4 to enhance the emission performance of g-C3N4. In addition, the ultraviolet absorption spectra of ZnONFs@PDA and sCuO showed considerable overlap with the ECL emission spectra of g-C3N4 appropriately. So sCuO and ZnONFs@PDA were combined and designed as an efficient dual-quencher of ECL luminescence of g-C3N4 by RET interaction. The biosensor showed superior linear detection range from 0.00005 to 50 ng mL-1, with a sensitive detection limit of 17.2 fg mL-1 (S/N = 3). It is worthy to note that a new type ECL-RET couple made up of g-C3N4-AuD@PPy (donor) and ZnONFs@PDA-sCuO (acceptor) was developed to construct a sandwich ECL biosensor for PCT detection. The studied immunosensor had satisfactory sensitivity, specificity and reproduc-ibility, indicating the proposed sensing method could provide a good technical means and theoretical basis for the diagnosis of serious diseases.
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