4.7 Article

CRISPR-Cas12a accessory cleavage activity triggering electrochemiluminescence biosensor for adenosine triphosphate detection

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biophysics

A rapid and ultrasensitive dual detection platform based on Cas12a for simultaneous detection of virulence and resistance genes of drug-resistant Salmonella

Xuran Fu et al.

Summary: This study developed a novel detection platform that can accurately and rapidly detect Salmonella and drug resistance genes with ultrahigh sensitivity. Cas12a-Ddp is not only suitable for rapid detection, but also applicable for detecting drug-resistant Salmonella in different liquid foods, with the potential for onsite application.

BIOSENSORS & BIOELECTRONICS (2022)

Article Biophysics

Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device

Tian Tian et al.

Summary: This study introduces a highly efficient dual-gene detection technique based on the orthogonal DNA/RNA collateral cleavage mechanism of the Cas12a/Cas13a system. The technique allows simultaneous detection of multiple amplified products and can be implemented on a portable device for accurate infectious disease screening in resource-limited settings.

BIOSENSORS & BIOELECTRONICS (2022)

Article Chemistry, Multidisciplinary

2′-O-Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR-Cas12a systems

Yuqing Ke et al.

Summary: In this study, we propose a strategy to enhance the specificity of the Cas12a system by using 2'-O-methyl modified guide RNA (gRNA), and we validate the effectiveness of this strategy through experiments. Our results show that the 2'-OMe modified gRNAs can significantly improve the specificity of Cas12a in discriminating single-base mutations, and molecular docking simulations reveal the mechanism of this enhancement.

CHEMICAL SCIENCE (2022)

Article Chemistry, Analytical

Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

Ahmed Mahas et al.

Summary: This article introduces a cell-free small molecule detection platform based on allosteric transcription factor (aTF)-regulated CRISPR array and Cas12a activity. The presence of a ligand leads to the dissociation of aTF, allowing transcription of the CRISPR array and activation of Cas12a collateral activity, resulting in increased fluorescence. Experimental results demonstrate the high sensitivity of the platform in detecting different tetracycline antibiotics using TetR as the aTF, and a hand-held visualizer and mobile phone application are used for fluorescence-based readout.

ANALYTICAL CHEMISTRY (2022)

Article Biophysics

A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics

Fei Hu et al.

Summary: A one-pot CRISPR/Cas13a-based visual biosensor was developed for rapid and low-cost nucleic acid detection. This method meets the need for rapid nucleic acid detection at home and on-site, and is suitable for resource-limited areas and epidemic outbreaks. The system has a limit of detection of 3 copy/μL and provides accurate results with shorter time and lower cost.

BIOSENSORS & BIOELECTRONICS (2022)

Article Chemistry, Multidisciplinary

Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems:Improvement Strategies and Performance Comparison

Reza Nouri et al.

Summary: This study developed a quantitative figure of merit (FOM) for CRISPR sensing to compare different methods and explore performance improvement strategies. By evaluating the FOM performances of existing studies, the effectiveness of strategies to improve FOM was found to be consistent with the model prediction.

ACS SENSORS (2022)

Article Chemistry, Multidisciplinary

Size-Controlled Engineering Photoelectrochemical Biosensor for Human Papillomavirus-16 Based on CRISPR-Cas12a-Induced Disassembly of Z-Scheme Heterojunctions

Yuxuan Li et al.

Summary: An innovative PEC biosensor based on quantum size-controlled engineering was developed for the detection of HPV-16. The quantum size effect was utilized to tune the photoactive material properties and optimize the PEC performance. The biosensor exhibited reliable detection of HPV-16 through experimental optimization.

ACS SENSORS (2022)

Article Chemistry, Multidisciplinary

Amplification of the Fluorescence Signal with Clustered Regularly Interspaced Short Palindromic Repeats-Cas12a Based on Au Nanoparticle-DNAzyme Probe and On-Site Detection of Pb2+Via the Photonic Crystal Chip

Yu-Yao Li et al.

Summary: A new DNAzyme chip for detecting Pb2+ in complex media includes the use of SNA fluorescence probe, Cas12a for sensitivity enhancement, and photonic crystal chip for accessibility, ultimately achieving low limit detection. These methods make Pb2+ detection more sensitive, specific, and promising for widespread applications.

ACS SENSORS (2022)

Article Chemistry, Analytical

Strand Displacement Amplification Assisted CRISPR-Cas12a Strategy for Colorimetric Analysis of Viral Nucleic Acid

Shaohua Gong et al.

Summary: The SDACC method reported in this study enables sensitive detection of viral nucleic acid, providing a simple and low-cost approach for colorimetric analysis.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Analytical

Aggregation-Induced Electrochemiluminescence Bioconjugates of Apoferritin-Encapsulated Iridium(III) Complexes for Biosensing Application

Lei Yang et al.

Summary: An AIECL bioconjugate was developed by encapsulating Ir(ppy)(3) complexes in apoFt, enhancing ECL emission and enabling sensitive detection of CYFRA 21-1. This study demonstrates the potential for utilizing apoFt in designing iridium(III)-based AIECL emitters for innovative ECL immunoassays.

ANALYTICAL CHEMISTRY (2021)

Article Biophysics

A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor

Yihan Yang et al.

Summary: This study presents a rapid, easy-to-implement, and accurate biosensing platform for detecting SARS-CoV-2 using CRISPR technology. By combining Cas13a collateral effect with HCR reaction, the method shows good discrimination capability when target RNA of SARS-CoV-2 is added to negative oropharyngeal swabs, demonstrating its promising potential for point-of-care diagnosis of COVID-19.

BIOSENSORS & BIOELECTRONICS (2021)

Article Chemistry, Multidisciplinary

A Superstable Luminescent Lanthanide Metal Organic Gel Utilized in an Electrochemiluminescence Sensor for Epinephrine Detection with a Narrow Potential Sweep Range

Cun Wang et al.

Summary: A novel lanthanide metal organic gel with electrochemiluminescence emission has been synthesized, showing stable and strong ECL signal for potential applications in electroanalytical chemistry. The gel exhibits promising performance in ECL intensity, allowing for sensitive detection of epinephrine with a linear range and low detection limit.

ACS SENSORS (2021)

Article Chemistry, Analytical

MXene Coupled with CRISPR-Cas12a for Analysis of Endotoxin and Bacteria

Anzhi Sheng et al.

Summary: In this study, it was found that MXene can efficiently adsorb single-stranded DNA and be coupled with CRISPR-Cas12a for sensitive detection of LPS and bacteria. Through carefully designed aptamers, the trans-cleavage activity of CRISPR-Cas12a is activated, allowing for selective and sensitive quantification of targets in different samples. This method provides a new direction for the exploration of universal analytical methods based on MXene coupled with CRISPR-Cas12a.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Analytical

Competitive electrochemiluminescence aptasensor based on the Ru(II) derivative utilizing intramolecular ECL emission for E2 detection

Qinqin Zhao et al.

Summary: This study investigated a competitive aptasensor for ultrasensitive detection of E-2 based on a novel intramolecular ECL emission system. By optimizing the Ru(II) derivatives and designing heterostructures as reaction accelerators, stronger ECL signals and further amplification effects were achieved in the detection of E-2.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

Article Chemistry, Analytical

CRISPR/Cas12a-Modulated fluorescence resonance energy transfer with nanomaterials for nucleic acid sensing

Xiaoxue Cheng et al.

Summary: This study explored the cleavage properties of Cas12a system on nanomaterial surfaces using gold nanoparticles and graphene oxide. The results indicated that graphene oxide exhibited better quenching ability and Cas12a-induced fluorescence recovery compared to gold nanoparticles, and double-stranded DNA probes with staggered ends led to superior cleavage efficiency.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

Article Chemistry, Multidisciplinary

Rotary Valve-Assisted Fluidic System Coupling with CRISPR/Cas12a for Fully Integrated Nucleic Acid Detection

Hui Wu et al.

Summary: A fully integrated nucleic acid detection system was established using a fluidic chip coupled with CRISPR/Cas12a, which achieved high sensitivity in nucleic acid extraction, amplification, and detection.

ACS SENSORS (2021)

Article Chemistry, Analytical

Novel Ratiometric Electrochemiluminescence Biosensor Based on BP-CdTe QDs with Dual Emission for Detecting MicroRNA-126

Jinwen Zhao et al.

Summary: The study utilized BP-CdTe QDs for single-emitter-based ECL ratio detection, achieving a low detection limit for miRNA-126 by using H2O2 and TPrA as dual co-reactants.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Physical

Modulating the 0D/2D Interface of Hybrid Semiconductors for Enhanced Photoelectrochemical Performances

Faying Li et al.

Summary: Photoelectrochemical solar-driven hydrogen production using semiconductors as active materials shows promising potential, but faces challenges like limited absorption range and rapid charge recombination. This study constructs a photoelectrode based on a 0D/2D heterojunction using metal chalcogenide quantum dots and hierarchical Zn-MoS2 nanosheets. By analyzing the effects of different quantum dot materials on the nanosheets, the researchers found that the 0D/2D heterostructure based on the composite Zn-MoS2/PbS@CdS significantly enhances the photocurrent due to synergistic effects and improved light harvesting capabilities.

SMALL METHODS (2021)

Article Chemistry, Multidisciplinary

Monitoring single Au38 nanocluster reactions via electrochemiluminescence

Mahdi Hesari et al.

Summary: The study reported for the first time single Au-38 nanocluster reaction events of highly efficient electrochemiluminescence (ECL) with tri-n-propylamine radicals as a reductive co-reactant at the surface of an ultramicroelectrode (UME). Statistical analyses of individual reactions confirm stochastic single ones influenced by the applied potential.

CHEMICAL SCIENCE (2021)

Article Nanoscience & Nanotechnology

Integration of Fe3O4 with Bi2S3 for Multi-Modality Tumor Theranostics

Keyi Luo et al.

ACS APPLIED MATERIALS & INTERFACES (2020)

Article Chemistry, Multidisciplinary

Luminescence Amplification at BiVO4 Photoanodes by Photoinduced Electrochemiluminescence

Jing Yu et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Chemistry, Multidisciplinary

CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

Ying Li et al.

CHEMICAL COMMUNICATIONS (2020)

Article Chemistry, Multidisciplinary

Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a

Peiwu Qin et al.

ACS SENSORS (2019)

Article Biochemistry & Molecular Biology

Broad-spectrum enzymatic inhibition of CRISPR-Cas12a

Gavin J. Knott et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2019)

Article Engineering, Environmental

Rapid extraction of uranium from sea water using Fe3O4 and humic acid coated Fe3O4 nanoparticles

Pallavi Singhal et al.

JOURNAL OF HAZARDOUS MATERIALS (2017)

Article Chemistry, Multidisciplinary

Aggregation-Induced Electrochemiluminescence of Platinum(II) Complexes

Serena Carrara et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Article Nanoscience & Nanotechnology

Fe3O4@polydopamine Composite Theranostic Superparticles Employing Preassembled Fe3O4 Nanoparticles as the Core

Rui Ge et al.

ACS APPLIED MATERIALS & INTERFACES (2016)