期刊
SENSORS
卷 22, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/s22207916
关键词
portable devices; fluorimetry; photometry; 3-D printing; easy fabrication; Arduino
资金
- AiF, Arbeitsgemeinschaft industrieller Forschungsvereinigungen Otto von Guericke e.V. [20964]
A novel portable low-cost Arduino-controlled photo- and fluorimeter has been developed for on-site measurements. The device demonstrates similar results to a laboratory spectrometer and fluorimeter in validation experiments, making it a viable option for field testing.
A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission light of the excited fluorescent dyes rhodamine 6G and calcofluor white was measured to validate the usability of the device as a fluorescence photometer. In all validation experiments, similar correlation coefficients and limit of detection could be achieved with the portable photo- and fluorimeter and a laboratory spectrometer and fluorimeter. Real sample analysis was performed, measuring phosphate concentration in freshwater and concentration of green fluorescent protein, extracted from Escherichia coli.
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