期刊
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
卷 398, 期 -, 页码 235-245出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jmmm.2015.09.004
关键词
Static magnetic field; Human adipose stromal/stem cells; Osteogenic differentiation
资金
- Wroclaw Research Centre EIT+ under the project Biotechnologies and advanced medical technologies - BioMed [POIG.01.01.02-02-003/08]
- European Regional Development Fund (Operational Programmed Innovative Economy, 1.1.2.)
- Wroclaw Centre of Biotechnology, program the Leading National Research Centre (KNOW)
- Foundation for Polish Science (FNP)
The aim of this work was to investigate the effects of static magnetic field (SMF) on the osteogenic properties of human adipose derived mesenchymal stem cells (hASCs). In this study in seven days viability assay we examined the impact of SMF on cells proliferation rate, population doubling time, and ability to form single cell derived colonies. We have also examined cells' morphology, ultrastructure and osteogenic properties on the protein as well as mRNA level. We established a complex approach, which enabled us to obtain informal:ion about SMF and hASCs potential in the context of differentiation into osteogenic and adipogenic lineages. We demonstrated that SMF enhances both viability and osteogenic properties of hASCs through higher proliferation factor and shorter population doubling Lime. We have also observed asymmetrically positioned nuclei and organelles after SMF exposition. With regards to osteogenic properties we observed increased levels of osteogenic markers i.e. osteopontin, osteocalcin and increased ability to form osteonodules with positive reaction to Alizarin Red dye. We have also shown that SMF besides enhancing osteogenic properties of hASCs, simultaneously decreases their ability to differentiate into adipogenic lineage. Our results clearly show a direct influence of SMF On the osteogenic potential of hASCs. These results provide key insights into the role of SMF on their cellular fate and properties. (C) 2015 Elsevier B.V. All rights reserved.
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