期刊
SCIENTIA HORTICULTURAE
卷 304, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scienta.2022.111246
关键词
Serine protease; Anthracnose; Postharvest; Loquat fruit; Disease resistance
类别
资金
- Program of Finance Department of Fujian Province in China [Nos.2015-1297, 2018-983, 2020-822]
- Science and Technology Planning Project of Fujian Province in China [2020N0001]
This study investigated the mechanism of keeping loquat fruit fresh using serine protease. The results showed that serine protease treatment inhibited the growth of Colletotrichum acutatum, enhanced the disease resistance of the fruit, reduced decay, and improved postharvest quality by regulating reactive oxygen species metabolism and activating defense systems.
Anthracnose caused by Colletotrichum acutatumis the most common disease-causing decay duringthe postharvest storage of loquat fruit.The use of serine proteasecould reduce decay of the fresh fruits during storage. However, the postharvest studies with the serine protease are limited. This study explored the mechanism of keeping loquat fruit fresh by serine protease. In vitro, SP treatment resulted in abnormal hyphae of C. acutatum, damaged plasma membrane integrity and decreased spore germination rate. Then we applied the best concentration of SP of 120 mg center dot L-1to explore its effect on loquat fruit. Test fruits were exposed to 2 different treatments, which are (i) serine protease (120 mg center dot L-1)+ C. acutatum, (ii)sterile water + C. acutatum as control. In vivo, SP treatment could regulate reactive oxygen species (ROS) metabolism of loquat fruit by enhancing the activities of catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX), and activating AsA-GSH circulatory system. SP treatment improved the disease resistance of loquat fruit to C. acutatum by enhancing the activities of phenylalanine ammonia lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO), chitinase (CHI) and beta-1,3-glucanase (GLU), promoting the accumulation of total phenolics and flavonoid. In addition, Spraying SP could increase the relative expression of genes related to fruit disease resistance (PAL, PPO, PR-1, TLP, ERF2, ERF4, WRKY22, GST). The results showed that the SP could inhibit the growth of C. acutatum, enhance the resistance of loquat fruit to pathogenic fungi, reduce postharvest decay, maintain postharvest quality and it was an effective biological preservative.
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